High-Purity Biotinylated Oligonucleotides Advanced Phosphoramidite Synthesis Technology
BOC Sciences offers biotinylated phosphoramidite synthesis service to your research specifications. Utilizing advanced technology, BOC Sciences can custom synthesize biotinylated nucleotides to support your molecular biology research needs. Biotinylated nucleotides are a valuable tool for DNA/RNA hybridization and PCR amplification, immunoassays, protein-nucleic acid interaction analysis, and many other molecular biology applications. As specialists in biotinylation chemistry and phosphoramidite synthesis, BOC Sciences can provide your biotinylated oligonucleotide synthesis with the highest purity and controlled modifications. Our team is experienced in both academic and industrial applications of biotinylated nucleotides and can produce biotinylated phosphoramidites quickly, reliably, and at a reasonable price. Contact BOC Sciences today to discuss your custom biotinylated phosphoramidite synthesis needs.
A biotinylated phosphoramidite is a modified nucleic acid phosphoramidite used for the synthesis of biotin-labeled oligonucleotides. The small, highly-affinity-binding molecule biotin is linked to the phosphoramidite functionality, which can then be used to incorporate biotin into nucleic acids. Biotinylated oligonucleotides have applications in molecular biology, diagnostics, and therapeutics.
Biotinylated phosphoramidites can be added to DNA or RNA oligonucleotide sequences with solid-phase synthesis methods. The phosphoramidite is bound to the backbone and the biotin is covalently attached to a specific location in the oligonucleotide. This creates a very efficient binding to streptavidin or avidin for use in many biotechnological applications.
Custom Synthesis of Biotinylated PhosphoramiditesWe provide custom synthesis of biotinylated phosphoramidites according to your research requirements. Our team can create modified phosphoramidites with biotin labels at specific positions and can also design specific oligonucleotide sequences with biotinylation. All our custom synthesis services are tailored to your exact needs, ensuring the highest quality and precision in every product.
High Purity Biotinylated Oligonucleotide SynthesisExperience the highest purity biotinylated oligonucleotide synthesis services. Our top-quality biotinylated phosphoramidites are perfect for accurately labeling your DNA or RNA sequences. Essential for precise and reproducible results in PCR, hybridization, and protein binding assays, our oligonucleotides are reliable and high-performing, ensuring your experiments achieve their full potential.
Biotinylation for Hybridization AssaysBoost the sensitivity and specificity of your hybridization assays (Southern blotting, Northern blotting, microarrays) with our biotinylated phosphoramidites. Our biotinylation services ensure efficient detection and capture of your target sequences, providing reliable and reproducible results in every experiment.
Biotinylated DNA/RNA for Protein Interaction StudiesAdvance your protein-nucleic acid interaction research with biotinylated DNA/RNA specifically designed for protein binding assays, ELISA, and other immunoassays. Our biotinylated DNA/RNA products provide high specificity for streptavidin and avidin, ensuring accurate and dependable results for your studies.
Technical Support and ConsultationGet expert technical support and consultation for your biotinylation and phosphoramidite projects. From assay optimization to troubleshooting, our team is here to help you every step of the way.
We provide a streamlined and efficient process for delivering high-quality biotinylated phosphoramidites tailored to your research needs. Our service is designed to be simple, fast, and reliable, ensuring you get the best results in your molecular biology applications.
To kickstart your project, our team offers an initial consultation to understand your specific requirements. We assess your research goals and help determine the exact specifications for the biotinylated phosphoramidite you need, including the oligonucleotide sequence, biotin positioning, and any additional modifications. This step ensures a customized solution that aligns with your research objectives.
Once your requirements are clear, our experts provide a custom synthesis proposal. This includes an outline of the biotinylated phosphoramidite synthesis plan, pricing, and an estimated timeline for delivery. We work with you to finalize the project details and ensure all aspects of the synthesis meet your expectations.
Our skilled chemists begin the synthesis process. Using state-of-the-art technology and high-quality reagents, we create biotinylated phosphoramidites with high precision and purity. We adhere to strict quality control protocols to ensure the final product meets the highest standards, ready for use in your experiments.
After synthesis, the biotinylated phosphoramidites undergo thorough quality assurance testing. We confirm the purity, yield, and proper biotinylation of the product. Our rigorous purification methods ensure that the phosphoramidites are free from impurities and ready for incorporation into your oligonucleotide sequences.
Upon successful completion and quality testing, we promptly ship the biotinylated phosphoramidites to your lab. We provide clear documentation, including purity certificates and usage guidelines, to ensure optimal results in your experiments. In case you need any assistance, our team is available for technical support throughout the process.
Once you receive the biotinylated phosphoramidites, we offer post-delivery consultation to assist with any additional questions or concerns. Whether you need help with protocol optimization or troubleshooting, our experts are available to provide ongoing support to ensure the success of your research.
Our biotinylated phosphoramidites are synthesized using state-of-the-art technology, ensuring the highest level of purity for your sensitive applications.
Tailor-made to meet your specific requirements, we provide custom biotinylated phosphoramidites in various lengths and functional modifications.
We understand the importance of time in research. Our team offers rapid synthesis and delivery, ensuring your projects proceed without delay.
Get the best value for your investment with our affordable pricing, backed by the quality and expertise you can trust.
Biotinylated phosphoramidites play an essential role in various research areas, including:
Enhance sensitivity and specificity in hybridization assays, including Southern blotting, Northern blotting, and microarray analysis.
Use in PCR amplification for capturing target sequences or enriching specific DNA fragments.
Employ biotinylated oligos in cell sorting, protein interaction studies, or receptor binding assays using biotin-streptavidin technology.
Improve detection in ELISA, Western blot, and immunohistochemistry by utilizing the high affinity between biotin and streptavidin.
Biotinylated phosphoramidites enable efficient biotin labeling of nucleic acids, which enhances their ability to bind to streptavidin or avidin. This high-affinity binding is crucial in applications such as molecular detection, purification, and quantification, making biotinylated oligonucleotides invaluable for gene analysis, protein interaction studies, and diagnostic assays.
We adhere to rigorous quality control protocols to ensure that every biotinylated phosphoramidite meets the highest standards. Each product undergoes purification, testing for purity, and biotinylation validation to guarantee consistency and accuracy. A certificate of analysis is provided with each order for full transparency.
Yes, we offer custom biotinylation at specific positions on your oligonucleotide sequences. During the consultation phase, we'll work with you to determine the best position for biotin incorporation based on your research needs and application.
