Enzyme Labeling of Proteins

* Please kindly note that our services can only be used to support research purposes (Not for clinical use).

BOC Sciences offers modified horseradish peroxidase (HRP), alkaline phosphatase (AP), and other substrate enzymes in purified, activated, and kit formats for easy conjugation to proteins and for the preparation of antibody conjugates for various immunoassays and related applications.

Introduction

Enzyme-protein conjugates, or cross-linked enzymes, a highly sensitive and specific molecular probe is prepared by connecting it to an antibody or antigen protein molecule in the form of covalent bond and ionic bond. It is widely used in analytical research and technical determination in the fields of medical pathology, immunohistochemistry, molecular biology, biopharmaceutical and veterinary medicine. A typical example is the binding of “activated” horseradish peroxidase (HRP) to target antibodies, proteins in immunoassay. On the other hand, as a carrier-free immobilization technology, the cross-linking of enzyme molecules can expand the industrial enzyme pool by increasing the stability during use and storage. Coupling reactions with proteins or peptides are usually carried out by small molecular connectors (such as glutaraldehyde) or enzymes (such as transglutaminase), also known as cross-linking.

Application

  • In research and laboratory detection, the modified enzyme horseradish peroxidase (HRP) is widely used in immunoassay applications.
  • It is spontaneously coupled with amines on antibodies and produces detectable signals in the presence of substrates.
  • In industry, the cross-linked enzyme produced by mixing the aqueous solution of the enzyme with the aqueous solution of glutaraldehyde is used to improve the productivity of the enzyme and avoid the cost of the carrier.

Our Advantages

  • Nearly 1:1 ratio of enzyme to interested target
  • Optimized sample preparation and conjugation
  • High product yield and purity
  • Strict validation and characterization
  • Scale-up production up to kilograms
  • Data analysis, detailed report with results and discussion
  • Analytical HPLC and MS analyses are performed in every development cycle.

Project Workflow

Project Workflow

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