Enzyme Labeling of Nanoparticle

As a leading service provider in drug discovery and research, BOC Sciences is committed to providing one-stop enzyme labeling services that have been applied to a wide range of assays and detection systems. With a comprehensive state-of-the-art platform, BOC Sciences offers enzyme nanoparticle coupling services to clients worldwide to facilitate your research.

What is Enzyme Labeling of Nanoparticles?

By coupling enzymes to nanoparticles, the catalytic activity of the enzyme can be combined with the physical properties of the nanoparticles to enable highly sensitive detection. Enzyme-labeled nanoparticles offer the advantages of high sensitivity, signal amplification, versatility, visual detection, high selectivity, as well as tunability and stability in bioanalysis and detection. This makes them an important tool for a wide range of applications in biomedical research, clinical diagnostics, environmental monitoring and biosensors.

Fig.1 Enzymatic bioconjugation of nanoparticles. (Walper et al., 2015)

Nanoparticle Enzyme Labeling Services

• Enzyme Labeling of Gold Nanoparticles
  Depending on customer needs, BOC Sciences can synthesize gold nanoparticles with specific size, shape and surface properties. The methods used to synthesize gold nanoparticles include chemical synthesis, wet chemistry, and sol-gel methods. And then, a suitable method is chosen to co-couple the enzyme with the gold nanoparticles so that the enzyme is immobilized on the surface of the gold nanoparticles.

• Enzyme Labeling of Magnetic Nanoparticles
  Customers can choose to provide magnetic nanoparticles and enzymes by themselves or customize the related products from us. We modify the magnetic nanoparticles and enzyme according to the customer's needs, so as to facilitate the expansion of their applications.

• Enzyme Labeling of Quantum Dots
  Quantum dots have a unique optical form and can be used in bioanalysis and microscopic imaging when combined with enzymes. In the process of labeling enzymes with quantum dots, we fully consider the emission wavelength of the quantum dots, the quantum yield, and the need to protect them to avoid affecting their function after synthesis need to be fully considered. The types of quantum dots available include carboxyl and amino water-soluble quantum dots, and oil-soluble quantum dots.

In addition, we also provide you with different types of enzymes labeling services, such as horseradish peroxidase (HRP), alkaline phosphatase (AP) and so on.

Enzyme-Functionalized Nanoparticles

• Enzyme Selection
  Enzyme selection needs to prioritize the availability of high catalytic activity, stability, and specificity. For example, a smaller molecular weight of the enzyme improves the spatial resolution of the labeling, while an enzyme with a longer half-life extends the duration of the labeling.

• Nanoparticle Functionalization
  Nanoparticles can provide high specific surface area, large loading capacity, and enhanced optical or magnetic properties. Suitable surface modifications can provide stable enzyme binding and maintain enzyme activity. Functional groups on the surface of nanoparticles are controlled and modified for efficient coupling to the enzyme.

• Enzyme Optimization
  Ensure that the enzyme activity is not damaged during the modification process, e.g., by avoiding extreme temperature, pH, and chemical conditions. In addition, optimize the interaction between the enzyme and the nanoparticles for proper conformation and catalytic activity of the enzyme.