BOC Sciences has developed a wide range of biotin labeled molecules, and will help you select the appropriate marker or labeling method and optimize the labeling method according to and for your specific needs. Biotinylation is the covalent binding of biotin to an antibody molecule through a chemical reaction. Biotinylated antibodies are mainly used in protein microarray analysis and EL immunohistochemistry. BOC Sciences' antibody labeling service offers a wide selection of labels and conjugates, providing customers with customized solutions.
Fig.1 Fc-specific biotinylation of antibody. (Yang et al., 2017)
High-specificity antibodies that have been biotinylated attach to target molecules to create stable complexes. The biotin antibody's target molecule or structure, to which it attaches, serves as a signal amplifier for detection. By introducing a biotin marker into the sample, the sensitivity of the detection signal can be enhanced by the binding of the biotin antibody, making the target molecule or structure easier to detect. In addition, biotin antibodies can be used in a variety of experimental techniques and applications, such as immunohistochemistry, immunoblotting, and immunoprecipitation. These techniques can be used to study protein expression, localization, interactions, etc., thus helping scientists to understand the functions and regulatory mechanisms of biological systems.
Biotin is dissolved in a suitable solvent and added to the antibody solution in a certain ratio for reaction. Free biotin and other reagents are then removed using dextran gel separation and purification/dialysis bags or ultrafiltration tubes, and the resulting antibody is stored in a suitable antibody preservation solution.
ELISA is a commonly used immunoassay technique to detect the presence and concentration of antibodies. By binding a labeled antibody to a specific antigen, ELISA can be used to measure the signal that binds to the antibody.
Flow cytometry can also be used to detect and quantify molecules on the surface of cells or within cells. In the detection of antibody concentration after labeling, fluorescently labeled secondary antibodies or other molecules that bind to the labeled antibody can be used to measure the intensity of the fluorescent signal in the cell by flow cytometry to infer the concentration of the antibody.
Generally, each antibody can be labeled with 3-5 biotin, and when labeling, the ratio of biotin to antibody is affected by the concentration of the antibody. For example, the biotin should be 12 times more than the protein for a 10 mg/mL antibody solution and 20 times more than the protein for a 2 mg/mL antibody solution. The biotin can also be added directly to the protein solution in the form of a powder. There are many different types of markers, and different types of markers have completely different properties, so leave this task to us. BOC Sciences offers a wide range of antibody labeling services.
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