In recent years, protein-DNA conjugates have been widely used to model self-assembling protein complexes in biological systems, such as assembly of enzymatic networks, highly ordered protein arrays, biomolecular delivery systems, etc. However, the high negative charge density of DNA can limit its practical application as a scaffold in self-assembled protein systems. As a DNA mimic, PNA has better stability than DNA. Unlike the DNA backbone, the skeleton of PNA can be altered by replacing glycine with different amino acids, such as lysine or cysteine, to provide active amine or thiol groups that allow PNA to bind specifically to proteins. Considering that PNA can efficiently conjugate to proteins through expressed protein ligations with high specificity, the self-assembled fluorescent protein system based on PNA conjugates has demonstrated a novel approach to controlling protein assembly. The unique structure and characteristics of PNA and the ability to study current model systems with fluorescence measurements make it a promising system for studying protein assembly under controlled conditions.
With our experts' expertise in click chemistry and years of experience in organic synthesis, BOC Sciences is able to offer coupling protein-PNA conjugation services that are unmatched in the industry.
Fig 1. Structural representations of protein, PNA, and DNA. (Tyler, B. M.; et al. 1999)
Protein-PNA conjugates hold great promise for the study of DNA-protein interactions, as well as being versatile molecular tools for biotechnology and materials science.
Fig 2. OaAEP1-mediated PNA-protein conjugation. (Zhangwei, L.; et al. 2021)
Biologically stable PNA chains can be used as a versatile tool for nucleic acid hybridization, and protein ligases can efficiently and site-specifically conjugate proteins to PNAs. At BOC Sciences, we employ strict expressed protein ligation (EPL) to covalently ligate proteins to peptide nucleic acids. This fast ligation is achieved by joining a short N-terminal GL dipeptide in the target protein to a C-terminal NGL tripeptide in the PNA.