Fluorescence Labeling of Antibody

* Please kindly note that our services can only be used to support research purposes (Not for clinical use).

Our custom fluorescent antibody labeling services combine years of conjugation expertise with a wide range of labeling options, including fluorescent dyes, phycobiliproteins and enzymes, as well as the selection of labeled chemicals. We provide conjugate services for research and commercial purposes.

Introduction

Immunofluorescence is a technique for fluorescent labeling of specific biological targets in samples using antibodies. Antibody is a Y-type high molecular glycoprotein, also known as immunoglobulin, which can bind specifically to other molecules (often referred to as antigens or epitopes). Fluorescent antibody labeling technique is to combine fluorescein with specific antibody by chemical method to form fluorescein-protein conjugate (fluorescent labeled antibody), which still retains antibody activity and has the tracer effect of fluorescein. When it was specifically bound to the corresponding antigen, it showed bright specific fluorescence with the help of fluorescence microscope.

Application

  • Isothiocyanyl fluorescent yellow (FITC) is commonly used as a marker in the experiment. In alkaline solution, the chemical group isothiocyanate (- N=C=S) on FITC binds to the free amino group of antibody protein (mainly the ε-amino group of lysine). Fluorescent antibodies were formed to determine the presence of the corresponding antigens in the cells.
  • Immunocytochemical (ICC) usually uses fluorescent labeled antibodies to study the subcellular distribution of proteins.
  • Cell permeable living cell dye, used in cell viability, ion, pH, nitric oxide, oxygen and stem cell culture.

Our Advantages

  • Deep knowledge and rich experience in biomaterial modification and conjugation
  • The labeling method is simple, safe and non-toxic
  • The fluorescence efficiency is high, and after binding to the protein, it can still maintain a high fluorescence efficiency
  • The binding of fluorescent markers to proteins does not affect the original biochemical and immune properties of proteins
  • The conjugate is stable and easy to preserve
  • Excitation and emission spectra for each product
  • Analytical HPLC and MS analyses are performed in every development cycle.
  • Identification of the quality of FITC markers by F value determination.
  • The coupling rate was determined by standard immunofluorescence staining test.

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