Fluorescence Labeling of Antibody
Fluorescently labeled antibodies are an important experimental tool that provides quantitative information about biomolecules at the cellular and tissue level, contributing to a deeper understanding of biological processes. With a wealth of experience and specialized scientists, BOC Sciences is able to bind your antibodies to a wide range of fluorescent reagents. BOC Sciences guarantees the delivery of antibodies labeled with fluorescent substances in a relatively short period of time.
What is Fluorescent Labeling of Antibodies?
Fluorescent labeling of antibodies involves attaching a fluorescent reagent to the antibody for targeted binding and visualization of specific molecules. When a fluorescently labeled antibody binds to a target molecule, the fluorescent dye emits a fluorescent signal at a specific wavelength, which can be observed and recorded by fluorescent devices. The combination of fluorescent labeling with antibodies allows researchers to track and study a variety of targets, including proteins, antigens and nucleic acids, on different experimental platforms.
Immunofluorescence is a technique for fluorescent labeling of specific biological targets in samples using antibodies. Antibody is a Y-type high molecular glycoprotein, also known as immunoglobulin, which can bind specifically to other molecules (often referred to as antigens or epitopes). Fluorescent antibody labeling technique is to combine fluorescein with specific antibody by chemical method to form fluorescein-protein conjugate (fluorescent labeled antibody), which still retains antibody activity and has the tracer effect of fluorescein. When it was specifically bound to the corresponding antigen, it showed bright specific fluorescence with the help of fluorescence microscope.
Fluorescently Labeled Antibody Services
BOC Sciences offers cutting-edge antibody fluorescent labeling services, providing researchers with tailored solutions to meet their specific experimental requirements. Focusing on quality and precision, BOC Sciences offers a range of services to meet different research needs.
(1) Selection of fluorescent labeling reagents
BOC Sciences offers a wide range of fluorescent labeling reagents, including fluorescein dyes and rhodamine dyes, which can be used for fluorescent labeling of a variety of molecules. By selecting a specific class of fluorescent reagent, we can help our customers complete their projects.
(2) Antibody labeling and conjugation
Based on the expertise and experience of BOC Sciences, we can provide our customers with a variety of technical methods for antibody labeling and conjugation. Various conjugation methods, including direct labeling, indirect labeling, and site-specific labeling, can be used to obtain highly efficient and stable antibody-dye conjugates.
(3) Quality control and characterization
All services and products provided by BOC Sciences require strict quality control procedures, and the prepared fluorescent antibodies need to be analyzed and characterized in various ways to ensure their labeling efficiency, stability, and functional integrity, as well as to ensure the reproducibility and scientific validity of the results.
Characterization Techniques for Fluorescently Labeled Antibodies
Fluorescence microscopy: Fluorescence microscopy allows visualization of fluorescently labeled antibodies at the cellular or subcellular level.
Flow Cytometry: By combining fluorescently labeled antibodies with flow cytometry, cells can be quantitatively analyzed and classified based on their fluorescent properties.
Immunohistochemistry (IHC) and Immunocytochemistry (ICC): IHC and ICC techniques utilize fluorescently labeled antibodies to detect and localize specific antigens within tissue sections or cultured cells, respectively.
Applications of Antibody Fluorescent Labeling
- Isothiocyanyl fluorescent yellow (FITC) is commonly used as a marker in the experiment. In alkaline solution, the chemical group isothiocyanate (- N=C=S) on FITC binds to the free amino group of antibody protein (mainly the ε-amino group of lysine). Fluorescent antibodies were formed to determine the presence of the corresponding antigens in the cells.
- Immunocytochemical (ICC) usually uses fluorescent labeled antibodies to study the subcellular distribution of proteins.
- Cell permeable living cell dye, used in cell viability, ion, pH, nitric oxide, oxygen and stem cell culture.
Our Advantages
- Deep knowledge and rich experience in biomaterial modification and conjugation
- The labeling method is simple, safe and non-toxic
- The fluorescence efficiency is high, and after binding to the protein, it can still maintain a high fluorescence efficiency
- The binding of fluorescent markers to proteins does not affect the original biochemical and immune properties of proteins
- The conjugate is stable and easy to preserve
- Excitation and emission spectra for each product
- Analytical HPLC and MS analyses are performed in every development cycle.
- Identification of the quality of FITC markers by F value determination.
- The coupling rate was determined by standard immunofluorescence staining test.
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