Fluorescence Labeling of Magnetic Nanoparticles
BOC Sciences offers fluorescence labeling technology, which combines fluorescent dyes with biomolecules for the visualization & detection of target molecules in life science research and biomedical applications. BOC Sciences' experienced scientists and technicians are able to select, optimize and validate the appropriate magnetic nanoparticles and fluorescent dyes according to the customer's needs and application areas, providing researchers and medical professionals with a powerful tool for the study of biomolecules, disease mechanisms and drug discovery.
Fig.1 Bioconjugation and fluorescence labeling of iron oxide nanoparticles. (Qiao et al., 2018)
Fluorescent Magnetic Nanoparticles
By combining fluorescent dyes with magnetic nanoparticles, the magnetic properties of the magnetic nanoparticles and the optical properties of the fluorescent dyes can be utilized simultaneously. The stability and biocompatibility of magnetic nanoparticles make them ideal for labeling and detection in biological samples. Magnetic nanoparticles have great advantages in biological fields such as magnetic resonance imaging, drug loading and cell detection, allowing the labeled target molecules to be guided and manipulated by an external magnetic field, which can be used to localize, isolate and enrich the target molecules. Meanwhile, the fluorescent dye allows the target molecules to be directly observed and analyzed under a fluorescent microscope or other detection equipment, providing the ability to visualize and quantify them. Most fluorescent magnetic nanoparticles have core-shell structure. The shell must be biocompatible and non-immunogenic to prevent the agglomeration of particles while minimizing non-specific interactions with proteins, cells and other components of the biological medium.
Fluorescently Labeled Magnetic Nanoparticles Services
Magnetic Nanoparticle Preparation
Magnetic nanoparticle preparation is based on the customer's needs and the selection of appropriate magnetic materials and nanoparticles (factors to be considered include nanoparticle size, shape, and surface properties, etc.).
Surface Functionalization
The surface of magnetic nanoparticles needs to be functionalized to provide specific binding to target molecules. This can be achieved either chemically or through bioconjugation reactions. For example, appropriate functional groups can be introduced on the surface of the nanoparticles to allow specific chemical binding to the target molecule.
Fluorescently Labeled Magnetic Nanoparticles
Fluorescently labeled magnetic nanoparticles can be conjugated by introducing an active molecule to the surface of the nanoparticle and chemically conjugating it to a fluorescent dye.
Optimization and Validation
After fluorescent labeling, the magnetic nanoparticles need to be optimized and validated to ensure that the magnetic nanoparticles are successfully labeled.
Fluorescent Magnetic Nanoparticles for Biomedical
Imaging
Fluorescent magnetic nanoparticles (FMNPs) can be used for multimodal imaging, combining magnetic resonance imaging (MRI) and fluorescence imaging.The magnetic properties of FMNPs enable high-resolution magnetic resonance imaging, providing anatomical information, while fluorescence enables cellular or molecular imaging with high sensitivity and specificity.
Magnetothermal Effect
When exposed to an alternating magnetic field, FMNPs can generate heat, a phenomenon known as the magnetothermal effect. This property can be used for cancer treatment based on localized thermotherapy, where FMNPs are aimed at the tumor site to heat it and induce tumor cell death.
Magnetic Separation and Purification
The magnetic properties of FMNPs make them easy to isolate and purify from complex biological samples using magnetic separators. This property is valuable in a variety of applications such as isolating specific cells, purifying biomolecules, or removing contaminants from biological samples.
Advantages
- Magnetic nanoparticles are nontoxic and biodegradable
- Fluorescence reagent and probe are economical and safe
- Highly biocompatible and non-toxic
- Deep knowledge and rich experience in biomaterial modification and conjugation
- The probe is stable and can be used within two years after one labeling
- The experimental period is short, the results can be obtained quickly, the specificity is good, and the location is accurate
- All samples are carefully monitored for stability and characterized to ensure batch to batch consistency
Project Workflow
Reference
- Qiao R, et al. Bioconjugation and fluorescence labeling of iron oxide nanoparticles grafted with bromomaleimide-terminal polymers[J]. Biomacromolecules, 2018, 19(11): 4423-4429.