In the fields of biomedical research and therapy, antibody technology has always played a crucial role. As an emerging approach for antibody modification, antibody-peptide conjugation technology is gradually demonstrating its unique advantages and vast potential applications. BOC Cciences specializes in providing antibody-DNA conjugation services, utilizing advanced site-specific conjugation techniques and optimized processes to precisely combine antibodies with DNA fragments. Our services cover the entire process, from the selection of antibodies and DNA fragments, conjugation design, experimental operations, purification, to analysis, aiming to provide high-quality, customized antibody-DNA conjugates that meet diverse needs in disease diagnosis, drug delivery, gene therapy, immunotherapy, biosensors, imaging, and more.
Antibody-peptide conjugation technology uses chemical and biological methods to create covalent connections between specific peptide segments and antibody molecules which results in functional and property-specific complexes. Through this conjugation approach antibodies can acquire new functionalities while maintaining their original specificity and affinity capabilities which includes improved stability and half-life extension alongside better cellular uptake. The introduction of specific peptide segments into antibody Fc regions boosts antibody-dependent cellular cytotoxicity (ADCC). This technology has the following advantages:
Precise Modification: The antibody-peptide conjugation technology allows specific modifications of antibody molecules which avoid the non-specific alterations common in traditional chemical processes while maintaining antibody activity and stability.
Functional Enhancement: The function of antibodies can be significantly enhanced through the conjugation of specific peptide segments. Specific peptides enhance therapeutic outcomes by improving antibody interactions with immune cells.
Improved Stability: Peptide segments introduced into antibodies enhance their thermal stability and protease resistance which results in a longer half-life in vivo.
Diversity: The antibody-peptide conjugation technology allows multiple peptide segments to be combined which enables multiple functional integrations to create multifunctional antibodies.
Cancer Treatment: Antibody-peptide conjugation technology holds great promise in cancer therapy. By conjugating cytotoxic or immune-modulating peptide segments with antibodies, therapeutic effects can be enhanced while minimizing side effects.
Disease Diagnosis: Antibody-peptide conjugates can serve as tools for detecting biomarkers, improving the sensitivity and specificity of diagnoses.
Drug Delivery: Antibody-peptide conjugates can act as drug delivery vehicles, precisely delivering drugs to target cells or tissues, improving drug efficacy and safety.
Gene Therapy: Antibody-DNA conjugates can serve as carriers for gene therapy, precisely delivering therapeutic genes to target cells, enabling gene therapy for specific diseases.
Antibody Screening and Customization: We offer antibody screening services based on customer requirements, including screening for monoclonal antibodies, polyclonal antibodies, and recombinant antibodies. Additionally, we provide antibody customization services, where we design high specificity and high affinity antibodies based on the customer's provided antigen or gene sequence.
DNA Fragment Design and Synthesis: Based on customer needs and project goals, we design specific DNA fragments, such as gene sequences, aptamers, or other functional DNA sequences. We also provide DNA fragment synthesis services, ensuring that the synthesized DNA fragments are of high purity and specificity.
Conjugation Scheme Design: Based on the antibodies and DNA fragments provided by the customer, we design personalized conjugation schemes, including selecting suitable conjugation sites, conjugation methods, and linkers. We provide detailed conjugation scheme design reports to ensure the feasibility and effectiveness of the plan.
Purification and Analysis: We employ advanced purification techniques, such as gel filtration and ion exchange, to purify the conjugated products and remove unreacted antibodies and DNA fragments. We use various analytical methods, including SDS-PAGE, mass spectrometry, and HPLC, to characterize and validate the conjugated products, ensuring their quality and activity.
Quality Control and Validation: We perform comprehensive quality control and validation of the conjugated products, including testing for purity, specificity, stability, and other relevant parameters. We provide detailed quality control reports to ensure that the products meet customer requirements and standards.
Functional Validation: Based on customer needs, we conduct functional validation of the conjugated products, such as targeting ability, cellular uptake, therapeutic efficacy, and more. We provide detailed functional validation reports to ensure the effectiveness and reliability of the products in applications.
Stability Testing: We conduct stability testing on the conjugated products to evaluate their stability under different conditions, such as temperature, pH, and storage time. We provide stability test reports to guide the storage and use of the products.
Precise Modification: Our antibody-DNA conjugation technology platform allows for precise modification of antibody molecules, avoiding non-specific modifications typical of traditional chemical methods, thus ensuring antibody activity and stability.
Efficient Conjugation: Our platform uses advanced conjugation technologies to ensure efficient binding between antibodies and DNA fragments, improving the yield and quality of the conjugates.
Strong Stability: The antibody-DNA complexes obtained after conjugation exhibit good stability, maintaining their activity and function in complex biological environments.
Multifunctionality: By conjugating different DNA fragments, multiple functions can be integrated, such as targeted therapy, gene regulation, and cellular uptake, providing possibilities for multifunctional antibodies.
Wide Applicability: Our technology platform is adaptable to various types of antibodies and DNA fragments and can be customized to meet specific client needs, ensuring suitability for different application scenarios.
Stable Linker Technology: Our platform employs various types of linkers, including cleavable and non-cleavable linkers. These linkers have high stability in circulation, preventing premature release of small molecule toxins, thus reducing systemic toxicity and enhancing drug safety.
Rapid Purification and Analysis: We possess advanced purification and analytical techniques, enabling quick purification and characterization of conjugates, ensuring their quality and activity. This provides strong support for clients' R&D and production.
Personalized Solutions: We offer personalized solutions based on specific client needs, including antibody and DNA fragment selection, conjugation site determination, linker design, etc. This personalized service ensures the success of clients' research and applications in antibody-DNA conjugation technology.
1. How do antibody-DNA conjugates work?
Antibody-DNA conjugates work by covalently linking antibodies with DNA fragments to form complexes with specific functions. The antibody is responsible for recognizing and binding to target molecules, while the DNA fragment can be used for detection, signal amplification, or carrying therapeutic payloads. For example, in immuno-PCR, antibody-DNA conjugates can be used to detect target molecules, achieving high-sensitivity detection through the amplification of DNA.
2. What are the methods for conjugating antibodies and DNA fragments?
Common conjugation methods include non-covalent binding (e.g., biotin-streptavidin binding) and covalent binding (e.g., thiol-maleimide chemical reaction). Additionally, there are some bioorthogonal reactions, such as iEDDA reactions, which enable fast and efficient conjugation.
3. What are the characteristics of the antibody-DNA complex after conjugation?
After conjugation, the antibody-DNA complex should maintain the specificity and affinity of the antibody while also possessing the DNA's amplification capacity and signal amplification function. Moreover, by designing suitable linkers, controlled release of DNA can be achieved, thus improving the sensitivity and specificity of detection.