BOC Sciences is a leading provider of siRNA Labeling services. With years of expertise in nucleic acid labeling technology, we offer customized solutions to meet your specific project requirements. Our dedicated scientific team uses first-hand knowledge and state-of-the-art technology to ensure superior results in labeling applications.
siRNA (small interfering RNA) labeling refers to the attachment of various tags (such as fluorophores, biotin, enzyme labels, etc.) to siRNA molecules by chemical or biological means. This labeling method allows researchers to track and analyze siRNA distribution, stability, uptake efficiency, and targeted gene silencing in and out of the cell. siRNA labeling technology has important application value in RNA interference (RNAi) research.
Commonly used fluorescent dyes include FITC, Cy3, Cy5, and Alexa Fluor, which can be used by fluorescence microscopy or flow cytometry to track and quantify siRNA distribution, transduction efficiency, and targeting effects in real time. Fluorescently labeled siRNAs facilitate the study of intracellular dynamics of siRNAs and their interactions with target mRNAs.
Biotin labeling is a highly sensitive and specific labeling technique. Detection and capture of siRNAs can be achieved by covalently attaching biotin molecules to siRNAs and then binding to streptavidin to form highly stable complexes. Biotinylated siRNAs are commonly used in experiments such as immunoprecipitation, affinity purification, and ELISA.
Enzyme labeling is the conjugation of enzymes (such as horseradish peroxidase, HRP) to siRNAs to achieve detection through chemiluminescence or chromogenic reactions. This method is simple to operate and has a significant signal amplification effect, and is often used in Western blot, immunohistochemistry, and other experiments.
Radiolabeling refers to the introduction of radioisotopes (such as 32P, 125I, etc.) into siRNA molecules in order to detect the presence and distribution of siRNAs by autoradiography or counters. This method is highly sensitive, but due to the dangerous nature of the use of radioactive materials, special caution is required in experimental handling and waste disposal.
Fig.1 Strategies of HEN1-directed labeling of small RNAs. (Plotnikova, Alexandra, et al., 2014)
The mechanism of action of siRNA labeling includes the following:
siRNA labeling technology enables researchers to observe the distribution and transduction efficiency of siRNAs within cells in real-time. Through fluorescence microscopy, the localization of siRNA in the cell can be visualized, which can help optimize the delivery system and method of siRNA and improve the efficiency of gene silencing.
siRNA labeling can help validate the targeting effect of siRNAs. By detecting the distribution of the labeled siRNA in a cell or tissue, it can be determined whether it has successfully reached the target region and exerts gene silencing. This is essential for preclinical research and evaluation of siRNA therapeutics.
Labeled siRNAs can be used to assess the transduction efficiency and in vivo distribution of different drug delivery systems, such as lipid nanoparticles, polymer-carriers, etc. By comparing the behavior of labeled siRNAs in different delivery systems, the optimal delivery vector can be screened to improve the therapeutic effect of siRNAs.
siRNA labeling technology can be used to study the interaction of siRNAs with their target mRNA, proteins, and other biomolecules. Through techniques such as co-immunoprecipitation, these interacting complexes can be captured and analyzed, providing insight into siRNA-mediated gene silencing mechanisms.
We offer a comprehensive range of siRNA labeling services, including design, synthesis, and characterization, to ensure high-quality labeled siRNAs to meet your research needs. Our services include:
Yes. Labeled siRNAs can be visualized in live cells to track endosomal escape, cytoplasmic distribution, and potential aggregation, providing insights into intracellular transport dynamics at a molecular level.
Different labels may subtly alter hybridization kinetics or steric accessibility. Studying these effects allows optimization of label types for accurate tracking without affecting gene-silencing functionality.
By using distinct fluorescent dyes on different siRNA species, researchers can simultaneously monitor multiple RNA molecules, investigate competitive interactions, and observe co-localization dynamics in complex cellular environments.
Factors like temperature, ionic strength, and buffer composition can affect labeled siRNA folding, stability, and cellular localization, offering a way to probe molecular dynamics under controlled experimental conditions.
Reference
