High-sensitivity High-stability Professional Antibody Labeling
We provide high-precision antibody HRP conjugation services for research, diagnostics, and industrial applications. Our expert team combines advanced periodate oxidation and cross-linking chemistry to covalently attach horseradish peroxidase (HRP) to your antibodies while maintaining optimal antigen-binding activity. Every conjugation is carried out under strictly controlled conditions to ensure maximum enzyme activity, low background noise, and high batch-to-batch consistency. From small pilot studies to large-scale production, we deliver purified, ready-to-use HRP-labeled antibodies that perform reliably in ELISA, Western blot, IHC, chemiluminescence, and colorimetric assays. With short turnaround times and full QC documentation, we help researchers and biotech companies accelerate their assay development and product validation.
Low signal or inconsistent assay performance: Poorly conjugated antibodies can lose enzymatic activity or binding affinity. Our optimized protocols guarantee high signal-to-noise ratios and reproducible results.
Time-consuming in-house conjugation: Avoid trial-and-error labeling steps and purification hassles. We deliver ready-to-use conjugates with verified activity.
Incompatible buffers and additives: We handle buffer exchange, desalting, and contaminant removal before conjugation to preserve both antibody and HRP activity.
Scalability and reproducibility issues: From R&D scale to production scale, every batch follows strict QC and activity testing standards.
Custom assay development needs: Our service supports diagnostic kit developers and CROs requiring consistent HRP labeling performance under regulatory conditions.
Custom Antibody-HRP Conjugation
Buffer Exchange and Sample Preparation
Poly-HRP Labeling for Enhanced Sensitivity
Quality Control and Validation TestingUV-Vis spectrophotometry for HRP-to-antibody ratio and activity verification.
SDS-PAGE electrophoresis to confirm conjugate purity and molecular integrity.
ELISA-based performance testing to ensure strong and specific antigen binding.
Ready-to-Use Conjugate Formulation
Bulk Production Service
We begin with a detailed discussion of your project requirements - including antibody type, concentration, target application (ELISA, WB, IHC, etc.), and desired labeling chemistry. Our technical team evaluates the optimal conjugation strategy and provides a clear quotation and turnaround timeline.
Upon receiving your antibody, we perform buffer compatibility checks, protein concentration measurements, and purity assessments to ensure it meets conjugation standards. If necessary, we conduct buffer exchange or sample cleanup prior to labeling.
Using our optimized periodate oxidation or cross-linking methods, HRP is covalently attached to the antibody under controlled conditions. Reaction parameters are precisely managed to preserve both enzymatic activity and antibody binding affinity.
The conjugate is purified using size-exclusion chromatography or ultrafiltration to remove free HRP and reaction byproducts. This ensures high-purity, stable conjugates suitable for direct downstream use.
Each batch undergoes comprehensive QC testing - including spectrophotometric ratio analysis, SDS-PAGE purity assessment, and ELISA-based functional validation - to confirm conjugation efficiency and antibody activity.
The final ready-to-use HRP-labeled antibody is shipped in a stabilized storage buffer with a complete QC report. Our support team provides documentation, storage guidelines, and technical consultation to ensure successful application in your assays.
All conjugations are performed by experienced chemists under optimized reaction conditions, ensuring consistent enzyme activity and reproducibility across batches. Each project follows a validated SOP with full traceability.
We employ both periodate oxidation and glutaraldehyde cross-linking to accommodate different antibody structures and applications. This flexibility ensures maximum HRP activity and stable enzyme-antibody bonding.
Our poly-HRP labeling and proprietary stabilization buffers enhance assay sensitivity, delivering strong signal-to-noise ratios for ELISA, Western blot, and chemiluminescent detection systems.
Each conjugate is validated through UV-Vis spectroscopy, SDS-PAGE, and functional ELISA testing. You receive a complete QC data sheet, ensuring confidence in every batch you use or commercialize.
We offer rapid service timelines (as fast as 5-7 business days) for small-scale projects, and scalable production up to gram quantities for OEM and diagnostic manufacturing needs.
Our scientists offer post-delivery technical consultation, assisting with assay optimization, troubleshooting, and performance validation. You're supported from project initiation through real-world application.
You should provide the antibody concentration, buffer composition, desired labeling chemistry (if any), target application (e.g., ELISA or IHC), and required final volume or format. Our team will assess feasibility and recommend the best conjugation approach.
These additives interfere with HRP coupling reactions. However, we offer buffer exchange and purification services to remove incompatible components before conjugation.
Each batch undergoes UV-Vis absorbance analysis, SDS-PAGE purity check, and ELISA-based activity validation. A complete QC report is included with every delivery.
Yes, we also provide biotin, FITC, alkaline phosphatase (AP), and fluorophore conjugation upon request for multiplex assay or dual-label applications.
Accelerate your research or diagnostic development with our professional antibody HRP conjugation services. Whether you need small-scale custom labeling or large production, our experts ensure high enzyme activity, reproducible performance, and rapid turnaround - all backed by comprehensive QC validation. Contact us today to request a quote, discuss your project specifications, or get personalized technical support. Let's transform your antibodies into high-performance HRP conjugates that drive reliable, publication-ready results.
