Peptide Glycosylation
BOC Sciences is a biological products and services provider offering a wide range of biotechnology services including peptide glycosylation. Our professional researchers are familiar with the latest research advances in the field of peptide glycosylation, and are able to provide professional advice and solutions to our customers. What's more, through quality control measures, we ensure that our clients receive satisfactory results.
Fig.1 Preparation of glycosylated peptides by direct and convergent strategies. (Moradi et al., 2016)
Glycosylated Peptides
Peptide glycosylation is a common post-transcriptional translational modification that occurs after protein synthesis. It can be carried out in an enzyme-catalyzed or non-enzyme-catalyzed manner. Enzyme-catalyzed peptide glycosylation occurs in the Golgi apparatus and endoplasmic reticulum and usually involves the concerted action of a series of enzymes. These enzymes are able to link the synthesized peptide chains with sugar molecules to form glycosylated peptides or glycosylated proteins. Peptide glycosylation plays an important biological function in organisms. It can affect protein folding, stability, cellular localization, and interactions with other molecules. Glycosylation can also regulate protein degradation rates, signaling and immune recognition.
Advantages of glycosylated peptides:
- Regulate the physical and chemical properties of peptide drugs and improve their absorption through biofilms.
- Glycosylated peptides can target specific organs.
- Enhance biological distribution in tissue.
- Improve permeability through biofilm.
- Increase metabolic stability and decrease clearance and receptor binding.
- Protect the side chains of amino acids from oxidation and maintain and stabilize the physical properties of peptides.
Peptide Glycosylation Services
BOC Sciences, as a provider of glycan conjugation services, offers glycosylated peptides services, which include preparation and analysis of glycosylated peptides, and peptide glycosylation engineering services, etc. BOC Sciences is able to efficiently provide customers with products while offering a range of product analysis and testing services.
- Services for Synthesizing Glycosylated Peptides
BOC Sciences provides services for the synthesis of glycosylated peptides, including the synthesis of peptide chains with specific glycosylation sites and the attachment of sugar molecules to the corresponding amino acid residues.
- Peptide Glycosylation Analysis
a. Glycosylation specific antibody detection
BOC Sciences detects glycosylated peptides using specific antibodies, which can be achieved by techniques such as immunoblotting, immunohistochemistry and immunofluorescence staining.
b. Glycosylation zymography
Glycosylation zymography allows for the excision of glycosylated modifying enzymes using a glycosylase enzyme to produce a deglycosylated peptide. The deglycosylation product is then analyzed by mass spectrometry or other methods to determine the glycosylation site and type of glycosylation.
c. Glycosylation site prediction and bioinformatics analysis
Amino acid sequences of proteins or peptide chains are analyzed and predicted by bioinformatics tools and algorithms to identify potential glycosylation sites. These tools assess the likelihood of glycosylation of amino acid residues and provide probabilities for site prediction.
- Peptide Glycosylation Engineering Services
Peptide glycosylation engineering services offered by BOC Sciences include glycosylation site modulation, glycosyltransferase engineering, substrate engineering, and metabolic engineering.
a. Glycosylation Site Regulation - Through genetic engineering, amino acid residues in glycosylation sites are changed to specific glycosylation sites, or non-glycosylation sites are changed to glycosylation sites. This can modulate the glycosylation modification sites of peptides and change their glycosylation pattern.
b. Glycosyltransferase Engineering - Genetically engineering the expression or activity of a glycosyltransferase enzyme to alter the type and extent of peptide glycosylation.
c. Substrate Engineering - Modulation of the structure and sequence of a substrate peptide by genetic engineering to alter its substrate specificity for glycosyltransferases.
d. Metabolic Engineering - Influencing the substrate supply and metabolic pathways for glycosylation modifications by modulating metabolic pathways and metabolite synthesis.
Advantages
- Development of therapeutic peptides using different glycosylation strategies
- High product yield and purity
- Strict validation and characterization
- Superior technical support: assist client from design to delivery
- Scale-up production up to kilograms
Project Workflow
Reference
- Moradi S V, et al. Glycosylation, an effective synthetic strategy to improve the bioavailability of therapeutic peptides[J]. Chemical science, 2016, 7(4): 2492-2500.