Biotin Labeled Magnetic Nanoparticles

BOC Sciences offers biotinylated magnetic nanoparticles, including ferrite nanoparticles, iron oxide nanoparticles and so on. We also offer a wide range of functionalized superparamagnetic particles for biomolecular separation, imaging and diagnostic applications.

Fig.1 Chemical structures of biotin/thionine modified magnetic microbeads. (Kuramitz et al., 2012)

Biotinylated Magnetic Nanoparticles

Magnetic nanoparticles have a highly sensitive magnetic signal that can be manipulated and detected by an external magnetic field. By labeling biotin onto magnetic nanoparticles, this highly sensitive signal can be combined with the specific binding of biomolecules to achieve highly sensitive detection of low concentrations of target molecules. Biotin-labeled magnetic nanoparticles can be further modified with other molecules or functional groups for additional applications. For example, fluorescent dyes, drug molecules, or other biomolecules can be labeled onto the magnetic nanoparticles to enable multimodal detection, localization, and treatment of target molecules.

Services for Biotinylated Magnetic Beads

First, magnetic nanoparticles such as ferrite (Fe₃O₄) or iron oxide (γ-Fe₂O₃) nanoparticles are synthesized. These nanoparticles usually have a small size (usually between 10-100 nm) with high saturation magnetization strength.

Next, the synthesized magnetic nanoparticles are surface modified to introduce biotin functional groups. This can be achieved chemically, e.g., by introducing compounds containing biotin functional groups on the surface of the nanoparticles or by immobilizing biotin on the surface of the nanoparticles via cross-linking agents.

Finally, a target molecule (e.g., a protein, nucleic acid, or cell) is bound to the biotin-labeled magnetic nanoparticle. The biotin binding sites in the target molecule bind to the biotin-labeled magnetic nanoparticles to form a stable complex.

Types of Biotinylated Magnetic Nanoparticles

• Biotinylated ferrite oxide nanoparticles
• Biotinylated iron oxide nanoparticles
• Biotinylated magnetic alloy nanoparticles
• Biotinylated other magnetic metal oxide nanoparticles

Optimization of Biotinylated Magnetic Nanoparticles

Magnetic nanoparticles can also be surface modified before biotin labeling. For example, fluorescent groups, antibodies, oligonucleotides, etc. are introduced to provide richer functionality.

In addition, the size and shape of magnetic nanoparticles have an important impact on their performance. By optimizing the synthesis method and reaction conditions, the size and shape of magnetic nanoparticles can be controlled to meet the needs of specific applications.

Application of Biotinylated Magnetic Beads

• Magnetic separation
• Isolation of avidin protein-labeled proteins and nucleic acids
• Study of protein interactions and DNA- protein pull-down
• Purification of avidin protein-labeled proteins and nucleic acids

The sample to be detected is pre-treated so that the target molecules can be more easily captured and enriched by the magnetic nanoparticles in the sample. Afterwards, the biotin magnetic nanoparticles are added directly to the sample so that they bind specifically to the target molecules. Then, through the external magnetic field, the magnetic nanoparticles bound to the target molecules can be separated and enriched. Finally, the target molecules can be detected by different methods, such as immunodetection (e.g., immunoblotting, immunofluorescence detection), nucleic acid hybridization, and mass spectrometry.

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