Gold Nanoparticles labeled Oligonucleotide
Gold Nanoparticles Labeled Oligonucleotide is the combination of gold nanoparticles with oligonucleotide molecules for the visualization or detection of oligonucleotides. This labeling method is commonly used in biomedical research, biosensing and drug delivery. Gold nanoparticles have unique optical and electrochemical properties, exhibiting strong absorption and scattering of light in the near-infrared region.
BOC Sciences specializes in providing customers with oligonucleotide modification services, especially gold nanoparticle labeling oligonucleotide services. When gold nanoparticles are combined with oligonucleotides, the sequence and structure of the oligonucleotides can be detected by changes in the optical properties induced by the surface plasmon resonance effect of the gold nanoparticles.
Oligonucleotide Modified Gold Nanoparticles for Intracellular Gene Regulation
Oligonucleotide modified gold nanoparticles can be used for intracellular gene regulation. This approach combines the unique properties of gold nanoparticles with the ability of oligonucleotides to interact specifically with target genes. The following is the flow of the method:
Synthesis of gold nanoparticles - Oligonucleotide design - Oligonucleotide affixation to gold nanoparticles - Characterization and purification - Cellular delivery - Gene regulation - Evaluation and analysis.
Gold Nanoparticles Labeled Oligonucleotide Services
Oligonucleotide Synthesis Services
Oligonucleotides with specific sequences can be synthesized based on gene sequences or RNA molecules provided by customers.
Specific Oligonucleotide Labeling Service
Gold nanoparticles are combined with the oligonucleotide to be labeled using appropriate chemical methods.
Characterization Analysis
The labeled oligonucleotide-gold nanoparticle complexes are characterized and analyzed by techniques such as UV spectroscopy, dynamic light scattering and transmission electron microscopy.
How Gold Nanoparticles Perform Oligonucleotide Labeling
Metal nanoparticles can be used as an effective biomarker for the labeling of oligonucleotides. The following is a common method called metal nanoparticle labeling technique with the following steps:
Step 1 - Synthesizing metal nanoparticles
Select metal nanoparticles of appropriate size and shape such as gold, silver or other metals. There are various methods for synthesizing metal nanoparticles, and common ones include chemical reduction method, solvent heat method, and so on.
Step 2 - Surface modification
The surface of the metal nanoparticles is modified in order to bind to the oligonucleotide molecules. The most commonly used modifiers are sulfhydryl compounds such as mercaptoethanol (2-mercaptoethanol) or mercaptopropionic acid (3-mercaptopropionic acid). The sulfhydryl groups are modified to the surface of the nanoparticles through the coordination of the sulfhydryl groups with the metal ions on the surface of the metal nanoparticles (e.g., gold ions).
Step 3 - Activation of sulfhydryl groups
Activate the sulfhydryl groups by adding an activator (e.g., N-hydroxysuccinimide, NHS) or using a cross-linker (e.g., glutaraldehyde). This will allow the sulfhydryl group to bind to the carboxyl group in the oligonucleotide molecule.
Step 4 - Labeling the Oligonucleotide
Covalently bind the oligonucleotide to the activated sulfhydryl group. The carboxyl group in the oligonucleotide molecule reacts with the sulfhydryl group to form a stable amide bond.
Step 5 - Washing and purification
Unbound oligonucleotides and other impurities are removed through a washing step. Common washing methods include centrifugation, ultrafiltration, or magnetic separation.
Advantages of Combining Oligonucleotides with Gold Nanoparticles
Enhanced detection of biomolecules
Gold nanoparticles have strong surface plasmon resonance absorption in the visible range, and their color can be modulated according to the size and shape of the particles. Therefore, combining oligonucleotides with gold nanoparticles can enhance the sensitivity and specificity of detection of biomolecules.
Enhanced biocompatibility
Gold nanoparticles are highly biocompatible nanomaterials that can be used in living organisms. By combining oligonucleotides with gold nanoparticles, biomolecules can be directed into cells or tissues and achieve specific targets.
Enhanced stability and durability
Gold nanoparticles have excellent chemical stability and physical durability and can maintain their functionality and shape under varying conditions for long periods of time. This makes gold nanoparticle-labeled oligonucleotides more convenient for experimental manipulation and storage.
Our advantages
- Stable and reliable product quality
- Excellent one-stop service
- Advanced instruments and equipment
- Analytical HPLC and MS analyses are performed in every development cycle.
- Guaranteed Quality: All samples are carefully monitored for stability and characterized to ensure batch to batch consistency.
- More flexible product packaging
- After-sales service is guaranteed (if there is any quality problem, you can return or exchange)
Project Workflow
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