In biomedical research and diagnostics, precise and efficient detection technologies are key drivers of scientific advancement. We provide peroxidase-labeled antibody technology services, leveraging an advanced technology platform, an experienced team, and a rigorous quality control system to deliver efficient, precise, and reliable antibody modification solutions to researchers and medical institutions worldwide. From initial consultation to antibody labeling, quality control, and delivery, we ensure service excellence throughout the entire process, helping to elevate your research and diagnostic capabilities.
Peroxidase-labeled antibody technology involves chemically conjugating highly catalytically active peroxidases (typically horseradish peroxidase, HRP) with specific antibodies. This conjugation preserves the antibody's high specificity and affinity while endowing it with the powerful catalytic colorimetric capabilities of peroxidase.
When the labeled antibody binds to its corresponding antigen under appropriate reaction conditions, the peroxidase catalyzes a colorimetric reaction of a colorless or colored substrate, generating soluble or insoluble colored products. This enables precise antigen or antibody localization and quantitative detection. Compared to traditional detection methods, peroxidase-labeled antibody technology offers several significant advantages:
High Sensitivity: The extremely high catalytic efficiency of peroxidase amplifies even weak antigen-antibody reaction signals, enabling precise detection of target molecules at very low concentrations. This greatly enhances detection sensitivity, allowing researchers to capture subtle biological changes.
Excellent Stability: The optimized labeling process ensures a strong and stable bond between the peroxidase and antibody, maintaining activity and specificity over long-term storage and experimental use. This reduces experimental errors due to reagent degradation or performance fluctuations, ensuring reliability and reproducibility.
Broad Applicability: Our peroxidase-labeled antibody products cover antibodies from various species and target common antigen epitopes, making them suitable for diverse research and clinical testing applications, including cellular immunology studies, histopathological analysis, and trace antigen detection in biological fluids.
Rapid and Convenient: Peroxidase-catalyzed colorimetric detection reactions typically proceed at high speeds, significantly shortening detection times. This supports efficient research and diagnostics, especially in clinical applications requiring rapid results.
Immunohistochemistry (IHC): Precisely localizes and detects specific protein expression in tissue sections, providing visual and reliable data for pathological diagnosis, disease mechanism research, and drug target screening. Peroxidase-labeled antibody technology enables clear visualization of antigen distribution within tissues, assisting pathologists in assessing disease severity and type, and informing clinical treatment decisions.
Western Blot (WB): A fundamental technique for detecting the relative molecular weight and expression levels of specific proteins in samples. Peroxidase-labeled antibodies enable specific binding to target proteins in Western blot experiments, allowing precise detection through chemiluminescence or colorimetric reactions. This facilitates the analysis of protein expression differences, post-translational modifications, and protein-protein interactions.
Enzyme-Linked Immunosorbent Assay (ELISA): A high-throughput, highly sensitive immunoassay widely used in clinical diagnostics, biopharmaceutical quality control, and quantitative antigen/antibody detection in research. Peroxidase-labeled antibodies bind specifically to antigens or antibodies coated on microplates, generating quantifiable signals upon substrate addition, providing an efficient and accurate solution for large-scale sample testing.
Other Immunodetection Applications: Includes immunofluorescence, immunoprecipitation, and other methods where peroxidase-labeled antibody technology plays a crucial role, offering flexible solutions for diverse research and diagnostic needs.
Antibody Selection and Customization: Based on customer-provided antigen information or target protein sequences, we utilize advanced antibody screening technologies to identify high-specificity, high-affinity antibodies from extensive libraries. We also offer monoclonal or polyclonal antibody customization tailored to client needs.
Antibody Purification and Preprocessing: Using affinity chromatography, ion-exchange chromatography, and other advanced purification techniques, we achieve high-purity antibody isolation from customer-provided or in-house produced antibodies. We remove impurities such as proteins and nucleic acids, ensuring purity standards for peroxidase labeling. Additionally, we perform essential preprocessing steps, such as buffer exchange and concentration adjustment, to optimize antibody conditions.
Peroxidase Labeling: Employing well-established chemical crosslinking or immunoadsorption methods, we precisely conjugate peroxidase to antibodies, carefully controlling labeling ratios and reaction conditions to maintain antibody activity and stability. We support multiple peroxidase options to meet different experimental requirements.
Labeled Antibody Purification and Quality Control: Post-labeling, we use gel filtration and affinity chromatography to remove unbound peroxidase, free antibodies, and other impurities. The purified labeled antibodies undergo strict quality testing, including enzyme activity assays, specificity validation, and purity assessment, ensuring outstanding product performance.
Expert Antibody Modification Team: Our experienced team specializes in antibody labeling, mastering various advanced labeling techniques. We provide customized peroxidase-labeled antibody services tailored to specific client needs, ensuring superior product quality and performance.
Stringent Quality Control System: From raw material procurement to final product release, we implement a rigorous quality control process, managing each step meticulously. Utilizing advanced international testing equipment and standards, we comprehensively assess the specificity, sensitivity, and stability of peroxidase-labeled antibodies to meet or exceed industry standards.
Fast Turnaround Time: Recognizing the time-sensitive nature of research and clinical work, we continuously optimize production processes to shorten delivery cycles. For standard peroxidase-labeled antibody products, we ensure rapid dispatch to meet urgent customer needs. For customized products, we complete production and quality testing in the shortest time possible, ensuring timely research progress.
Comprehensive After-Sales Support: We offer extensive technical support and after-sales services, with expert teams available to provide consultations and troubleshooting assistance. Additionally, we provide product usage training and experimental protocol optimization to help customers maximize the effectiveness of our peroxidase-labeled antibody technology for optimal experimental results.
1. How to Order Peroxidase-Labeled Antibody Services?
You can contact us via phone, email, or online customer service to inquire about our services. Our professional technical team will discuss your specific requirements and tailor a personalized service plan. Once both parties confirm the plan, a service contract will be signed. After the payment of a deposit, we will officially initiate the project.
2. What Types of Antibodies Can You Label?
We can label various types of antibodies, including but not limited to:
Monoclonal antibodies
Polyclonal antibodies
Antibodies derived from different species, such as mouse, rabbit, goat, and more.
Additionally, we provide customized services, including antibody screening, purification, and pre-treatment, according to client requirements.
3, Can You Label Antibodies with Low Purity?
Yes. Before labeling, we purify the provided antibodies using advanced purification techniques such as affinity chromatography and ion exchange chromatography. This ensures the antibody meets the required purity for peroxidase labeling by removing impurities like non-specific proteins and nucleic acids, optimizing its condition for successful labeling.
4. How Do You Ensure the Activity and Specificity of the Labeled Antibody?
We employ well-established chemical crosslinking or immunoadsorption methods to label peroxidase to antibodies, carefully controlling the labeling ratio and reaction conditions. This ensures that the antibody's activity and specificity are not compromised during the labeling process. After labeling, we use purification techniques such as gel filtration and affinity chromatography to remove any unbound peroxidase, free antibodies, and other impurities, guaranteeing optimal performance of the labeled antibodies.
5. Can You Label Different Types of Peroxidases?
Yes. We offer a selection of peroxidase labeling options, including:
Horseradish peroxidase (HRP)
Alkaline phosphatase (AP)
Clients can choose the most suitable peroxidase based on their specific experimental system and research needs.
6. Do You Provide Quality Control Reports?
Yes. We provide a detailed quality control report with every order, ensuring transparency and reliability of the labeled antibodies.