Gold Nanoparticles labeled Streptavidin
BOC Sciences offers a full range of custom nanoparticle coupling services, including antibodies, DNA, RNA, biotin and oligonucleotides. Our biomarker services are tailored to your requirements, ensuring efficient and precise labeling while maintaining the integrity and functionality of the biomolecules involved.
Streptavidin Conjugated Gold Nanoparticles
Streptavidin modified gold nanoparticles can bind biotinylated proteins or oligonucleotides. Streptavidin-biotin system is characterized by high sensitivity, good specificity, high stability and wide applicability, etc. One streptavidin protein can bind multiple biotin molecules, so the amplification system of streptavidin-modified gold nanoparticles and biotinylated proteins can be combined to detect trace amounts of proteins and oligonucleotides.
Gold Nanoparticles labeled Streptavidin Service
BOC Sciences provides professional nanoparticles & beads coupling service, based on our rich experience and technology, we can provide customized service for streptavidin-modified gold nanoparticles. Our nanoparticle labeling service provides a comprehensive and efficient solution for the development and production of bioconjugates.
- Design of streptavidin
In addition to Streptavidin, BOC Sciences offers other types of affinity couplings, such as Avidin or Neutravidin, which have different properties and affinities and can be selected according to experimental purposes and conditions. Moreover, through rational sequence design and mutation, the sequences of the affinities can be optimized so as to enhance the binding ability of the affinities to their receptors and reduce non-specific binding.
In addition, to facilitate detection and labeling, tagging sites, such as fusion tags (e.g., His tags or GST tags) or fluorescent tags (e.g., GFP or RFP), can be introduced into the sequence of the affinity pigments.
- Modification of gold nanoparticles
The surface of gold nanoparticles can be modified by introducing functional groups or modifying molecules. Common functional groups include sulfhydryl group (-SH), amine group (-NH2), carboxyl group (-COOH), and so on. And the modifications include the formation of gold-sulfur bonds between sulfhydryl groups and gold atoms on the surface of gold nanoparticles, or the coupling reaction of amine and carboxyl groups using cross-linking agents (e.g., EDC/NHS). Alternatively, by adsorption binding, certain proteins or peptides can be bound to the gold nanoparticle surface by electrostatic interactions or hydrophobic interactions.
- Synthesis of gold nanoparticles labeled streptavidin
BOC Sciences specializes in preparing streptavidin conjugated gold nanoparticles according to your specific requirements, and our labeling services are competitively priced and of the highest quality. We also offer a wide range of modification and purification methods for gold nanoparticles.
- Product scale-up
BOC Sciences provides comprehensive analytical support to ensure the success of the coupling process of gold nanoparticles to streptavidin. We employ a variety of techniques such as HPLC, LC-MS, and NMR to confirm successful coupling and assess the purity and integrity of the final product. Additionally, with both small and large volume infrastructure and production capabilities, we can accommodate projects of varying sizes, from small research needs to large scale production needs, ensuring flexibility and scalability.
Application of Streptavidin Conjugated Gold Nanoparticles
- Gold nanoparticles labeled streptavidin can be used for the secondary detection of biotinylated probes in detection methods such as western blot, ELISA, light microscope and electron microscope.
- Gold nanoparticles labeled stretavidin are increasingly used in biosensors. For example, stretavidin-coated gold nanoparticles are modified with biotinylated oligonucleotides, which can improve the sensitivity of nucleic acid tests.
- A gold nanocluster immunoassay (GNCIA) has been developed based on streptavidin-labeled fluorescent gold nanoclusters for early and sensitive detection of HIV infection.
Our Advantages
- Excellent one-stop service
- High quality fluorescent conjugates of gold nanoparticles and streptavidin with different sizes
- Highly reliable and reproducible result
- Data analysis, detailed report with results and discussion
Project Workflow
