BOC Sciences offers a full range of custom nanoparticle coupling services, including antibodies, DNA, RNA, biotin and oligonucleotides. Our biomarker services are tailored to your requirements, ensuring efficient and precise labeling while maintaining the integrity and functionality of the biomolecules involved.
Streptavidin modified gold nanoparticles can bind biotinylated proteins or oligonucleotides. Streptavidin-biotin system is characterized by high sensitivity, good specificity, high stability and wide applicability, etc. One streptavidin protein can bind multiple biotin molecules, so the amplification system of streptavidin-modified gold nanoparticles and biotinylated proteins can be combined to detect trace amounts of proteins and oligonucleotides.
BOC Sciences provides professional nanoparticles & beads coupling service, based on our rich experience and technology, we can provide customized service for streptavidin-modified gold nanoparticles. Our nanoparticle labeling service provides a comprehensive and efficient solution for the development and production of bioconjugates.
In addition to Streptavidin, BOC Sciences offers other types of affinity couplings, such as Avidin or Neutravidin, which have different properties and affinities and can be selected according to experimental purposes and conditions. Moreover, through rational sequence design and mutation, the sequences of the affinities can be optimized so as to enhance the binding ability of the affinities to their receptors and reduce non-specific binding.
In addition, to facilitate detection and labeling, tagging sites, such as fusion tags (e.g., His tags or GST tags) or fluorescent tags (e.g., GFP or RFP), can be introduced into the sequence of the affinity pigments.
The surface of gold nanoparticles can be modified by introducing functional groups or modifying molecules. Common functional groups include sulfhydryl group (-SH), amine group (-NH2), carboxyl group (-COOH), and so on. And the modifications include the formation of gold-sulfur bonds between sulfhydryl groups and gold atoms on the surface of gold nanoparticles, or the coupling reaction of amine and carboxyl groups using cross-linking agents (e.g., EDC/NHS). Alternatively, by adsorption binding, certain proteins or peptides can be bound to the gold nanoparticle surface by electrostatic interactions or hydrophobic interactions.
BOC Sciences specializes in preparing streptavidin conjugated gold nanoparticles according to your specific requirements, and our labeling services are competitively priced and of the highest quality. We also offer a wide range of modification and purification methods for gold nanoparticles.
BOC Sciences provides comprehensive analytical support to ensure the success of the coupling process of gold nanoparticles to streptavidin. We employ a variety of techniques such as HPLC, LC-MS, and NMR to confirm successful coupling and assess the purity and integrity of the final product. Additionally, with both small and large volume infrastructure and production capabilities, we can accommodate projects of varying sizes, from small research needs to large scale production needs, ensuring flexibility and scalability.
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The process involves conjugating streptavidin molecules to gold nanoparticles via a covalent bonding technique, ensuring stable attachment for further use in experiments.
Yes, gold nanoparticles labeled with streptavidin are ready to use after appropriate storage, but it's recommended to check the concentration and ensure they are well-dispersed before use.
Gold nanoparticles labeled with streptavidin are compatible with most standard laboratory buffers and conditions. However, it's important to avoid harsh chemicals that might disrupt the conjugation.
Labeling efficiency can be assessed using UV-Vis spectroscopy to confirm the presence of streptavidin on the gold nanoparticle surface, ensuring proper conjugation.
