As a leading service provider in the field of drug discovery and research, BOC Sciences is fully competent and dedicated to serving one-stop molecule labeling services which has be used in various detection and assay system. With the comprehensive advanced platform, BOC Sciences provides global clients with carbohydrate-oligonucleotide conjugation services to promote your research.
This conjugation can be performed at the 5'-end of the oligonucleotide sequence, using modified phosphoramidites. First, oligonucleotide scaffolds were synthesized using commercially available and modified phosphoramides on solid carriers using a DNA synthesizer. After the first "click" reaction with the galactosylated azide derivative on the solid carrier, the bromine atom is replaced by the azide compound and reacts with propargyl mannose on the solid carrier or in solution. In addition, using mono-alkynated solid carriers, it is possible to bind to carbohydrate residues at the 3'- end of oligonucleotides according to a similar scheme.
In recent years, carbohydrate-oligonucleotide conjugates are becoming more and more important, especially in the uptake and cell utilization of siRNA and antisense oligodeoxynucleotides. Of particular interest here is N-acetylgalactosamine (GalNAc), which binds to the desialoglycoprotein receptor (ASGPR) in hepatocytes.
