Stability Enhancement Reduced Immunogenicity Extended Half-life Targeted Delivery
Boost the performance of your biologics with our advanced PEGylation services. We specialize in site-specific PEGylation to enhance drug stability, prolong half-life, reduce immunogenicity, and improve solubility. Our customizable solutions ensure optigh-quality, scalable PEGylation solutions that accelerate drug development and regulatory success.
Fig. 1 PEG-prodrug. (Mishra, P., 2016)The chemical structure of Polyethylene Glycol (PEG) is HO-(CH2CH2)n-OH, which is a general term for ethylene glycol polymers with relative molecular mass between 200-8000 and above. PEG has precise molecular weight and is colorless, tasteless, non-toxic, non-immunogenic, biocompatible, highly soluble in water, high purity and low dispersibility.
PEGylation is the process of covalently attaching polyethylene glycol (PEG) chains to therapeutic molecules—such as proteins, peptides, antibodies, or nanoparticles—to enhance their pharmacokinetic and pharmacodynamic properties. This proven bioconjugation technique improves solubility, increases half-life, reduces immunogenicity, and enables targeted drug delivery, making it a vital tool in modern drug development and biologics formulation.
Supply of PEG Reagents and Raw Materials
PEGylation Experimental Operations and Process Development
Purification and Separation of PEGylated Products
Characterization and Analysis of PEGylated Products
Custom PEGylated Product Development PartnershipsWe provide expert PEGylation design and custom conjugation strategies tailored to your specific biomolecule—whether it's a protein, peptide, antibody, or nucleic acid. Our services include comprehensive analysis of molecular structure and functional groups, selection of the most suitable PEG type (linear, branched, or multi-arm), and identification of optimal PEG molecular weight. We employ a variety of proven conjugation chemistries, including NHS ester and maleimide coupling, to ensure site-specific and efficient modification. Each project is supported by optimized reaction conditions and scalable protocols to maximize bioactivity, stability, and in vivo half-life—enhancing drug performance and therapeutic outcomes.
Enhance nuclease resistance and in vivo stability with our PEGylated oligonucleotide conjugation services for improved therapeutic delivery.
Extend antibody half-life and reduce immunogenicity with site-specific PEG conjugation optimized for targeted biotherapeutics.
Improve solubility and stability of amino acid-based compounds through precise PEG conjugation tailored for research and drug design.
Boost carbohydrate bioconjugate performance with PEGylation to enhance pharmacokinetics and reduce immune recognition.
Increase protein drug stability, solubility, and circulation time with our expert protein PEGylation services for biologic development.
Achieve sustained peptide activity and reduced degradation via high-efficiency PEG conjugation techniques.
Enable targeted drug delivery and improved circulation with PEG-coated nanoparticles engineered for enhanced bioavailability.
Small Molecule PEG ConjugatesImprove solubility, circulation time, and therapeutic index of small molecule drugs with our precision PEGylation services for optimized drug delivery.
PEGylated Lipid ConjugatesEnhance lipid nanoparticle stability and stealth properties with our tailored PEG-lipid conjugates for efficient mRNA and gene therapy delivery.
Enzyme PEG ConjugatesExtend enzyme half-life, reduce immunogenicity, and improve therapeutic performance with our enzyme-specific PEGylation solutions.
PEGylated Vaccine AntigensStabilize and modulate immune response with PEGylated vaccine antigens engineered for enhanced efficacy and reduced reactogenicity.
PEGylated RNA Delivery SystemsBoost RNA stability and delivery efficiency using our PEGylated carriers and lipid-PEG systems designed for advanced nucleic acid therapeutics.
Aptamer PEG ConjugatesIncrease aptamer bioavailability, nuclease resistance, and target-binding efficiency with our high-purity PEG conjugation platforms.
Viral Vector PEG ConjugatesEnhance viral vector stealth, circulation, and tissue targeting with our PEGylation strategies tailored for gene therapy applications.
Exosome/EV PEG ConjugatesImprove extracellular vesicle stability and reduce immune clearance using our surface PEGylation techniques for next-gen delivery platforms.
We have extensive experience in custom PEGylation and comprehensive expertise in chemical and biological preparations.
Our PEGylation services can be applied to a wide range of products such as proteins, peptides, antibodies, and small molecules, making us a versatile partner in the biopharmaceutical industry.
We offer custom synthesis services and modify the properties of molecules according to customer-specific requirements.
Our proven processes and efficient workflow ensure rapid turnaround times without sacrificing quality or accuracy.
We adhere to stringent quality regulations and standards to ensure utmost product consistency, potency, and purity.
We fully respect our clients' intellectual property, maintaining strict confidentiality and offering transparent, clear communication throughout the entirety of each project.
Despite our high-quality services, we offer competitive pricing models that prove cost-effective for our customers.
We pride ourselves on our exceptional customer service, where we maintain constant communication and provide progress updates throughout each project.
Many top-tier biopharmaceutical companies around the world have chosen to partner with us, reflecting our reliability and professional expertise in PEGylation technology.
Our company has expertise in PEGylation technology and we assure to provide robust, efficient and reliable solutions tailored to your specific needs.
PEGylation can be utilized to improve the pharmacokinetics of drugs. By attaching a PEG molecule to a therapeutic compound, its circulation time in the blood can be prolonged, reducing the frequency of administration and improving patient compliance. PEGylated drugs demonstrate decreased immunogenicity and antigenicity, increased solubility, and enhanced stability.
PEGylation is used to reduce non-specific binding in diagnostic tests. Non-specific binding can lead to false-positive results and by PEGylating the diagnostic biomarkers, the accuracy of test results can be improved.
PEGylation is also used in various biotechnology applications such as protein and peptide stabilization. By attaching PEG molecules to proteins and peptides, their stability, solubility, and resistance to proteolytic degradation can be increased.
The technique of passive targeting drug delivery relies primarily on the concentration gradient produced between intracellular and extracellular spaces due to the drug's high concentration in the tumor vicinity. PEG conjugates take advantage of the enhanced permeation and retention (EPR) effect triggered by tumors to accumulate in the tumor vessels' pathological environment, facilitated by leaky vasculature and deficient lymphatic drainage. However, this effect is size-dependent and cannot be examined with low molecular weight drugs that can easily extravasate and cause systemic toxicity. The process of PEGylation enhances the size, molecular mass, solubility, and serum stability of drugs. Consequently, PEGylation is highly recognized as one of the most effective methods for passive targeting of anticancer treatments for these significant reasons.
Fig. 2 A schematic illustration of passive targeting with acid-sensitive PEG-prodrugs that cleave in the extracellular space. (Mishra, P., 2016)
The early stages of creating PEG-protein conjugates relied on reactions with limited chemoselectivity which non-specifically altered the surface nucleophiles of proteins. However, advancements in bioorthogonal reactions and the utilization of unnatural amino acids in proteins have allowed for improved control over PEGylation site and degree. Some techniques include reductive alkylation for N-terminal a-amino groups, targeting Cys residues with PEG maleimides, vinyl sulfones, disulfides, or iodoacetamides, or converting Cys side-chains into dehydroalanine for modification with PEG thiol. Despite the possibility of diastereomeric mixtures, the significance of this stereochemical inconsistency hasn't been experimentally explored. Additionally, unnatural amino acids containing orthogonally reactive functional groups can be integrated into the proteins themselves and then selectively modified with corresponding PEG reagents. This produces PEG-protein conjugates with precisely-positioned PEGs, which opens up new possibilities for protein-based therapeutics.
Fig.3 Examples of natural and unnatural amino acids used in protein-PEG conjugation. (Paul, B.L., 2016)
PEGylation is the process of attaching the Polyethylene Glycol (PEG) polymer chains to another molecule, typically a drug or therapeutic protein, which can improve the safety and efficacy of therapeutic agents.
PEGylation can enhance the therapeutic properties of drugs by increasing their stability, solubility and half-life, improving their pharmacokinetics, and reducing immunogenicity and toxicity.
By increasing drug solubility, stability and reducing the clearance rate, PEGylation prolongs the circulation time of the drugs in the body, leading to a longer duration of action and better drug delivery to the targeted tissues or cells.
As a stealth polymer, PEG can effectively shield the drug molecule from the immune system, reducing the immunogenicity and antigenicity, which minimizes the risk of immune responses and side effects.
PEGylation may lead to the loss of biological activity of some proteins, but with the optimal design of PEGylation strategy, this negative impact can be minimized or offset by the improved pharmacokinetic properties.
Yes, we offer site-specific PEGylation strategies to control the PEGylation site and ratio, aiming to maximize the therapeutic effects and minimize potential side effects.
A wide range of molecules can be PEGylated, including small molecule drugs, peptides, proteins, antibodies, oligonucleotides, and other therapeutic agents.
We have established strict quality control systems, including the analysis of PEGylation degree, homogeneity, and bioactivity, to ensure the high quality and batch-to-batch consistency of PEGylated products.
We provide comprehensive PEGylation services, from PEGylation strategy design, custom synthesis of PEGylated products, to analytical services such as PEGylation degree analysis and bioactivity test.
