Gold Nanoparticles labeled Protein

At BOC Sciences, we have a dedicated team, technology, and experience and follow a strict quality control system to expedite the development of our clients' projects. BOC Sciences offers services for gold nanoparticle labeling of protein.

Functionalized Gold Nanoparticles

Functionalized gold nanoparticles are a class of nanomaterials that endow gold nanoparticles with new properties and functions by introducing specific functional groups or molecules (e.g., proteins) on the surface of the gold nanoparticles. Gold nanoparticles have many unique physical, chemical, and optical properties that make them important nanomaterials for a wide range of applications in biomedicine, sensors, catalysts, optical materials, and other fields. By functionalizing gold nanoparticles, the range of applications and functions can be further extended and adapted to specific applications.

Fig.1 Synthesis schematic of transporter protein WGA-HRP and drug THP conjugated to the AuNPs. (Zhang et al., 2016)

Conjugation of Proteins to Gold Nanoparticles

There are multiple modes of interaction between gold nanoparticles and proteins, including physical adsorption, chemical covalent binding, and non-covalent specific adsorption. Click chemistry can covalently connect gold nanoparticles and proteins, or catalyze the reaction between nanoparticles, to obtain reaction results that can be read with the naked eye. The interaction between gold nanoparticles and proteins and the spectral properties of gold nanoparticles can be used for simple, efficient and low-cost detection of disease or environmental pollution.

Gold Nanoparticles Labeled Protein Service

Gold nanoparticles interact with proteins in a variety of ways, including physical adsorption, chemical covalent binding, and non-covalent specific adsorption. BOC Sciences offers various protein labeling services.

• Gold Nanoparticles Labeling of Protein A
  Protein A is a protein on the surface of Staphylococcus aureus, with a molecular weight of 42kDa. It has 5 different structural domains that can bind to the Fc fragment of IgG and has a strong specific affinity. In performing the labeling process, gold nanoparticles need to undergo appropriate surface modifications, such as amination, carboxylation, or sulfation.

• Gold Nanoparticles Labeling of Protein L
  Protein L is a protein derived from the bacterium Lactobacillus and has the ability to bind the variable region of immunoglobulins. The binding of gold nanoparticles to Protein L can be achieved by methods such as physical adsorption or covalent binding. After the labeling is completed, we will evaluate its performance. Detection and quantification of the target protein can be achieved by binding gold nanoparticle-labeled Protein L to a specific antibody or immunoglobulin.

• Gold Nanoparticles Labeling of Protein G
  Protein G is a cell surface protein of group G Streptococcus with a molecular weight of 25 kDa, which binds specifically to the Fc region of IgG and also binds with low affinity to the Fab region. BOC Sciences offers customization, optimization, and scale-up of gold nanoparticle-labeled Protein G with guaranteed quality standards.

• Gold Nanoparticles Labeling of Albumin
  Albumin is one of the most abundant circulating proteins in plasma, accounting for half of the total plasma proteins in the normal human body. BOC Sciences can provide gold nanoparticle labeling of albumin and develop applications for the labeling of gold nanoparticles after the characterization of the product and evaluation of its properties. By analyzing the properties of the markers, the gold nanoparticles, albumin, and manufacturing processes are optimized for broader applications.

• Gold Nanoparticles Labeling of Transferrin

Transferrin is a protein involved in iron transportation and regulation in the body. BOC Sciences pre-treats the transferrin and gold nanoparticles before binding to ensure that their function is not altered upon binding.