Peptide Conjugation Services

At BOC Sciences, we specialize in providing comprehensive peptide conjugation services tailored to meet your specific research and development needs. With our expertise in bioconjugation chemistry and state-of-the-art facilities, we offer a wide range of conjugation options to modify peptides for various applications in drug delivery, diagnostics, and biotechnology.

Service Overview

• Labeling Services: Biotin Labeled Peptides, Enzyme Labeling of Peptides, Fluorescence Labeling of Peptides.
• Modification Services: Cholesterol-Conjugated Peptide, PEG Conjugated Peptide, Peptide Glycosylation.
• Conjugation Services: Peptide-Drug Conjugation (PDC), Peptide RNA Conjugation, Peptide Oligonucleotide Conjugation, Peptide-PNA Conjugation, Protein-Peptide Conjugation.
• Label-Free Conjugation Services: Protein/Peptide Crosslinking, Stable Isotope Labeling of Peptides.
• Functionalization Services: Peptide Liposome Conjugation, Peptide Conjugated Gold Nanoparticles.
• Analysis and Characterization Services: Mass Spectrometry Analysis, HPLC Analysis, Gel Electrophoresis.

Peptide Conjugates

We provide a comprehensive range of peptide conjugation services for various applications, including diagnostic assays, drug therapy, imaging, nucleic acid delivery, and affinity chromatography. Our service portfolios encompass a diverse array of peptide conjugates, including enzyme conjugates, antibody conjugates, nanoparticle conjugates, DNA/RNA-peptide conjugates, drug-peptide conjugates, and more.

• Peptide-Carrier Proteins Conjugates

We offer conjugation of peptides with a variety of immunogenic carrier proteins, including Keyhole Limpet Hemocyanin (KLH), Diphtheria Toxoid, and others. These conjugates can be utilized as immunogens or for affinity purification in research and diagnostic assays.

• Peptide-Drug Conjugates

Our peptide-drug conjugation services enable the covalent attachment of therapeutic agents such as drugs, biologics, or toxins to peptide delivery vectors. We specialize in conjugating drugs with short delivery vectors, including cell-penetrating peptides (CPPs), for targeted drug delivery applications.

• Other Peptide Conjugates

We offer coupling of peptides with various molecules, including enzymes, oligonucleotides, and small molecules, to enhance their properties for specific applications.

Peptide Bioconjugation

Peptide bioconjugation refers to the process of linking peptides to other biomolecules or synthetic compounds to create multifunctional conjugates with diverse applications. This process often involves the use of cross-linking reagents or reactive functional groups to facilitate the covalent attachment of peptides to target molecules. Bioconjugation techniques enable the creation of peptide-drug conjugates for targeted drug delivery, peptide-antibody conjugates for diagnostic assays, and peptide-nanoparticle conjugates for imaging and therapeutic applications.

1. Our peptide conjugation methods include N-terminal amine labeling, C-terminal acid labeling, incorporation of artificial amino acids, and coupling through functional groups such as Cys thiol.
2. Conjugates are purified using size-exclusion chromatography (SEC) or high-performance liquid chromatography (HPLC) to remove contaminants and ensure high purity.
3. Quality control measures include gel electrophoresis, mass spectrometry (MALDI MS), and other appropriate analytical techniques to verify the identity and purity of the conjugates.

Peptide Modification

In addition to peptide conjugation, we offer a wide range of peptide modification services, including labeling with fluorescent or enzymatic substrates, attachment of tags or labels, and incorporation of post-translational modifications (PTMs). BOC Sciences' custom peptide modification services allow for precise control over peptide characteristics and functionalities tailored to your specific requirements.

Peptide modification involves the chemical or enzymatic alteration of peptides to introduce specific functionalities or enhance their properties. Common modifications include the addition of fluorophores, chromophores, or other labels for imaging and detection purposes, as well as the incorporation of post-translational modifications (PTMs) to mimic natural peptide structures. Our expertise extends to the synthesis of modified peptides with precise modifications tailored to your requirements, including C-terminal modifications, N-terminal modifications, peptide cyclization, backbone modifications, labeling/conjugation.

Applications of Peptide Conjugation

1. Immunogen Design and Affinity Purification

Peptide conjugation with immunogenic carrier proteins, including Keyhole Limpet Hemocyanin (KLH) and Diphtheria Toxoid, facilitates the generation of immunogens for antibody production. These conjugates can also be used in affinity purification to isolate specific antibodies for research or diagnostic purposes.

2. Targeted Drug Delivery

Peptide-drug conjugation offers a promising approach for targeted drug delivery. By conjugating therapeutic agents with peptide delivery vectors, such as cell-penetrating peptides (CPPs), researchers can enhance drug uptake and specificity, potentially reducing off-target effects and improving therapeutic outcomes.

3. Diagnostic Assays

Peptide conjugation plays a vital role in diagnostic assays by enabling the immobilization of peptides onto surfaces or carriers for the detection of specific biomolecules. These conjugates can be labeled with fluorescent, chemiluminescent, or chromogenic substrates to facilitate sensitive and specific detection in various diagnostic platforms.

4. Research Tools

Peptide conjugates serve as valuable research tools for studying protein-protein interactions, gene regulation, and cellular signaling pathways. By conjugating peptides with nucleic acids, enzymes, or nanoparticles, researchers can develop innovative tools for studying complex biological processes with high precision.