Services
As a leading CRO, BOC Sciences specializes in offering top-notch bioconjugation services that cater to drug discovery and drug delivery. Our team of highly skilled scientists possesses vast expertise in an extensive range of bioconjugation techniques. Whether it's traditional cross-linking methods or click chemistry approaches, we have the proficiency to apply the most appropriate technique based on the unique requirements of each project. We invest in advanced spectrometers, chromatography systems, and other analytical instruments, ensuring accurate characterization and validation of bioconjugates.
What is Bioconjugation?
Bioconjugation is the process of linking two or more biomolecules together, often involving the covalent attachment of synthetic molecules to biological entities. These biomolecules can include proteins, antibodies, enzymes, nucleic acids, and more. The result is a bioconjugate, a novel hybrid entity with properties distinct from its individual components. Bioconjugation enables the introduction of desirable attributes, such as increased stability, targeted specificity, and enhanced bioactivity.
Bioconjugation Services
We understand that each research project is unique, and therefore, we offer customized solutions tailored to your specific objectives. Our team works closely with you to comprehend your research goals, design an optimal bioconjugation strategy. We take pride in our flexibility to accommodate various biomolecule types, conjugation chemistries, and project complexities. Our bioconjugation services include:
GMP Bioconjugation
We set up and operate a Good Manufacturing Practice (GMP) bioconjugation service, adherence to strict regulatory guidelines, and a commitment to quality and safety. Our GMP bioconjugation capabilities include regulatory compliance, personnel training and qualification, SOP development, equipment and instruments, raw materials and quality control, process validation, quality assurance and quality control, documentation and record keeping, facility audits and inspections, and continuous improvement.
Bioconjugation Techniques
Chemical Conjugation
- Disulfide Bond Formation
- EDC/NHS Coupling
- Maleimide Chemistry
- Aldehyde/Ketone Chemistry
Enzymatic Conjugation
- Transglutaminase catalyzes the formation of isopeptide bonds between specific amino acid residues.
- Sortase recognize specific peptide motifs and catalyze the formation of a peptide bond between the motif and a nucleophile.
Click Chemistry Bioconjugation
- Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC)
- Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)
- Strain-Promoted Alkyne-Nitrone Cycloaddition (SPANC)
- Tetrazine Ligation
- Staudinger Ligation
Biotin-Streptavidin Interaction
Biotin is a small molecule that forms an extremely stable and specific interaction with streptavidin.
Genetic Fusion/Expression
Genetic engineering techniques allow the fusion of specific peptide tags or functional proteins to the target biomolecule during its expression in cells or organisms.
Metal Chelation
Chelating agents, such as EDTA or DTPA, can be conjugated to biomolecules and subsequently complex with metal ions, enabling various applications in imaging and diagnostics.
Hydrophobic Insertion
Hydrophobic molecules can be inserted into lipid membranes or hydrophobic pockets of proteins to achieve bioconjugation.
Bioconjugate Analysis
We employ a variety of methods for characterizing and validating the success of bioconjugation reactions. Our comprehensive bioconjugation platform enable us to assess the efficiency of conjugation, determine the stoichiometry of the bioconjugate, and ensure the integrity and functionality of the resulting product.
- UV-Vis Spectroscopy
- Fluorescence Spectroscopy
- Mass Spectrometry
- Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS)
- Electrospray Ionization Mass Spectrometry (ESI-MS)
- Gel Electrophoresis
- Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
- High-Performance Liquid Chromatography (HPLC)
- Circular Dichroism (CD)
- Dynamic Light Scattering (DLS)
- Gel Filtration Chromatography
- Nuclear Magnetic Resonance (NMR) Spectroscopy
- Enzyme-Linked Immunosorbent Assays (Elisa)
- Zeta Potential Measurement
Bioconjugation Applications
Our bioconjugation services serve a wide range of clients in drug conjugates, drug discovery, and various other areas of research and industry.
- Therapeutic Antibodies and ADCs
- Drug Delivery and Targeting
- Nucleic Acid Delivery and Gene Therapy
- Vaccines and Immunotherapies
- Protein Labeling and Modification
- Glycoprotein Analysis
- Imaging and Diagnostics
- Biosensors and Assays
- Biomaterials and Tissue Engineering
- Nanotechnology and Nanomedicine
- Molecular Biology and Genomics
- Research Tools and Reagents
Why Choose BOC Sciences's Bioconjugation?
- We offer a full spectrum of molecules for bioconjugation: antibodies, peptides, proteins, fluorescent dyes, enzymes, oligonucleotides, nanoparticles, etc.
- Our labs boast sophisticated facilities meeting stringent quality standards.
- Our facilities accommodate projects of varying scales, catering to individual researchers and large-scale initiatives.
- Our meticulous validation procedures ensure reliable data for your research conclusions.
- We prioritize the confidentiality of your research and employ stringent measures to protect your data and intellectual property.
- With streamlined processes and efficient workflows, we deliver results within agreed timelines.