As a leading service provider in the field of drug discovery and research, BOC Sciences is fully competent and dedicated to serving one-stop labeling services which can be used in various detection and assay systems. With the comprehensive advanced platform, BOC Sciences provides fluorescence labeling service to promote your research.
Fluorophore is fluorescent chemical compounds that are highly conjugated and can re-emit fluorescence upon light excitation. Fluorophore can be used alone as a tracer, a probe or a substrate for enzyme. While covalently bonding with macromolecules, it serves as a marker and help quantification and tracking of the biomolecules by fluorescent imaging. The non-constructive nature and high sensitivity of fluorescence technique makes fluorescent labeling one of the most advantageous method to track biomolecules in many ways.
A good fluorescent probe is usually small in size, chemically stable and not reactive to the biomolecules. The labeling should be efficient and adaptable to the target molecule establishing a covalent linkage between the probes and a specific residue in the target molecules.
There are several ways of labeling, including chemically synthesis, enzymatic reactions, and via tags. BOC Sciences is experienced with all kinds of labeling methods and can help with linkage design.
Fluorescent labeling scheme: a schematic diagram showing the labeling between a biomolecule (green) linked to a tag (light blue) and a fluorophore (navy blue)
Fluorescent Methods | ||
Chemical | Enzymatic | Tagging |
Maleimide-Thiol | Transglutaminase | Tetracysteine |
NHS ester-Amine | Sortase | Histidine |
Isothiocyanate-Amine | Cutinase | Aspartate |
Alkyl Halide-Hydroxy | Intein | Lanthanide |
Isocyanate-Hydroxy | Lipoic Acid Ligase | CLIP |
Tetrazine-Alkene/Alkyene | Biotin Ligase | Halo |
Myristoyl Transferase | SNAP | |
Fluorescent Proteins (Genetically Tagged) |
Our molecular marking services include experimental design, protocol and program development for the preparation of conjugates for a variety of detection and analysis systems. With novel technology platform and professional scientist team, BOC Sciences offers clients a wide range of Fluorescence labeling services, and we promise to provide customized solutions to meet each customer's unique requirements. Please feel free to contact us for more details.