Fluorescence Labeling

High-PrecisionNext-Level Fluorescent BioconjugationAdvanced Fluorescent Labeling Technologies

Accelerate breakthroughs with high-performance fluorescent labeling services engineered for organizations at the forefront of biotechnology, pharmaceuticals, diagnostics, and contract research. Built on state-of-the-art conjugation chemistry, optimized reaction systems, and strict quality control, our solutions deliver fluorescently labeled biomolecules with exceptional brightness, superior stability, and industry-leading reproducibility. From antibodies, proteins, peptides, and nucleic acids to lipids, nanoparticles, and small-molecule probes, we provide fully customized fluorescent labeling tailored to diverse research applications. Whether you are developing next-generation therapeutics, advancing diagnostic platforms, conducting cell imaging or molecular biology studies, or running high-throughput screening, our labeled biomolecules ensure the strongest signal integrity, minimal background, and consistent performance across experiments.

Choose from single-fluorophore labeling, multiplex fluorescent panels, or custom fluorophore integration designed for even the most demanding workflows. Every conjugate is engineered for maximum sensitivity, exceptional photostability, and reliable batch-to-batch consistency, empowering your team to generate confident, publication-quality results and streamline your research pipeline.

What Is Fluorescent Labeling?

Fluorescent labeling enables researchers to track, visualize, and quantify biomolecules using dyes that emit light upon excitation. By conjugating fluorophores to antibodies, proteins, peptides, nucleic acids, lipids, or small molecules, our service supports applications ranging from cell imaging and flow cytometry to HTS, mechanism studies, diagnostics, and drug-target interaction analysis. We specialize in custom, highly controlled labeling, ensuring your biomolecule maintains its bioactivity, purity, and specificity after conjugation.

What Problems We Solve

Weak Signals

We improve fluorescence intensity with optimized dye selection and controlled dye-to-molecule ratios, ensuring bright and reliable signal output.

Activity Loss

Our gentle, application-matched conjugation methods preserve protein, nucleic acid, and small-molecule functionality during the labeling process.

High Background

Free dye and impurities are removed through advanced purification, resulting in clean, low-noise fluorescence for imaging and quantitative assays.

Spectral Overlap

We design multi-color panels with precise spectral spacing to prevent signal interference and improve accuracy in flow cytometry and microscopy.

Stability Issues

Photostable dyes, optimized buffers, and controlled reaction conditions ensure strong signal retention and resistance to photobleaching.

Difficult Substrates

We successfully label challenging molecules—including hydrophobic drugs, lipids, oligos, and nanoparticles—where standard methods often fail.

Our Fluorescent Labeling Services

We provide a complete portfolio of custom fluorescent labeling services, covering biomolecules, nucleic acids, nanoparticles, lipids, enzymes, and specialty compounds. Each project is supported by precision chemistry, optimized conjugation strategies, and high-purity purification workflows to ensure exceptional brightness, stability, and biological integrity.

Fluorescence Labeling of Antibodies

Capabilities include:

Site-specific or random labeling via amine, thiol, glycan, or Fc-preserving chemistries
Controlled DAR (Degree of Labeling) optimization for consistent fluorescence performance
Compatible with IgG, IgM, IgA, Fab fragments, scFv
Specialized low-aggregation labeling ideal for flow cytometry, IHC, immunofluorescence
Buffer exchange and format optimization (PBS, Tris-free, BSA-free, azide-free)
Multi-color/multi-panel antibody labeling with spectral overlap minimization
High-purity conjugates prepared by SEC, desalting columns, or affinity cleanup
Fluorescent labeling compatible with diagnostic assay development
Low-endotoxin antibody preparations for sensitive cellular applications
Full QC panel including absorbance ratio, SDS-PAGE, fluorescence spectra, DOL, and optional binding activity testing

Popular dyes:

FITC, PE, APC, Alexa Fluor, Cy3, Cy5, Cy7, FAM, TRITC

Fluorescence Labeling of Proteins & Enzymes

Capabilities include:

Covalent conjugation using NHS esters, maleimide-thiol coupling, click chemistry (SPAAC, DBCO, TCO)
Labeling for soluble proteins, membrane proteins, enzyme complexes, and fusion proteins
Custom strategies to avoid active-site masking and maintain enzymatic function
Labeling densities adjustable for kinetic assays, fluorescence polarization, and biosensor applications
High-stability labeling for long-term imaging and high-intensity laser exposure
Purification via size-exclusion chromatography, HPLC, or ultrafiltration
QC support including intact mass verification, SDS-PAGE, fluorescence scan
Compatibility with FRET, FLIM, BLI, SPR, and high-throughput screening
Large-scale protein labeling for industrial research or assay kit production
Formulation development to improve solubility, reduce aggregation, and enhance stability

Popular dyes:

Alexa Fluor, FITC, TAMRA, TRITC, Cy3, Cy5, Bodipy, ATTO dyes, NIR dyes

Fluorescence Labeling of Peptides & D-Amino Acids

Capabilities include:

N-terminal, C-terminal, or internal site labeling with FAM, TAMRA, Cy dyes, Bodipy, etc.
Labeling compatible with linear peptides, cyclic peptides, disulfide-rich peptides, and stapled peptides
Custom linker/spacer engineering (C2–C12, PEGylated linkers) to reduce quenching
Ideal for receptor-binding assays, uptake studies, protease substrate studies
Purification via HPLC with >90–98% purity options
Fluorescent peptide libraries for screening and SAR studies
Labeling of D-amino acid–containing peptides for cell wall labeling or metabolic tracking
Dye-to-peptide ratio optimization for enhanced fluorescence readouts
High solubility formulations suitable for cell-based assays
Optional modifications: acetylation, amidation, PEGylation, lipidation

Popular dyes:

FAM, TAMRA, ROX, Cy3, Cy5, Bodipy, Rhodamine, Alexa Fluor

Fluorescence Labeling of Nucleic Acids

(DNA, RNA, siRNA, Oligos, dNTPs)

Capabilities include:

5', 3', or internal labeling of DNA, RNA, siRNA, miRNA, and aptamers
Synthesis and labeling of fluorescent dNTPs, dUTP, ddNTPs, and modified nucleotides
Production of FAM-labeled siRNA, Cy5-labeled oligos, and dual-labeled qPCR probes
Custom quencher + reporter combinations for FRET and molecular beacons
High-purity HPLC/PAGE purification and mass confirmation
Integration of spacers (C6, C12, PEG linkers) for improved hybridization performance
Labeling optimized for cellular uptake, trafficking, and live-cell RNA imaging
Validated compatibility with qPCR, NGS library prep, hybridization assays, in vivo tracking
Custom duplex, hairpin, or modified backbone support (LNA, 2'-OMe, phosphorothioate)
High-stability fluorophores for long-term imaging and low background noise

Popular dyes:

FAM, HEX, ROX, Cy3, Cy5, ATTO dyes, Alexa Fluor

Fluorescent Labeling of Small Molecules

Capabilities include:

Covalent labeling of small-molecule drugs, fragments, scaffolds, metabolites, and chemical probes
Multiple chemistries supported: NHS esters, maleimide, click chemistry (SPAAC, DBCO, TCO–tetrazine), sulfonyl chlorides
Labeling compatible with hydrophobic, charged, and aromatic-rich compounds
Custom linker insertion to tune solubility, permeability, and reduce steric hindrance
Purification using HPLC, prep-HPLC, or flash chromatography with >95% purity
Ideal for target engagement assays, HTS, fluorescence polarization, and binding kinetics
Custom fluorophore integration for FRET probes, biosensor probes, and enzyme substrates
Mass-spec validated products with optional NMR confirmation
Optimization for in-cell or in vivo imaging of small-molecule behavior
Formulation support for DMSO-based stocks or aqueous-compatible delivery

Popular dyes:

FAM, TAMRA, Cy3/Cy5, Bodipy, Rhodamine, Alexa Fluor

Fluorescent Labeling of Lipids & Phospholipids

Capabilities include:

Labeling of phospholipids, glycolipids, sphingolipids, cholesterol derivatives, and lipid analogs
Incorporation of membrane-compatible fluorophores such as BODIPY, NBD, DiI, DiO, ATTO dyes
Covalent attachment or membrane insertion labeling depending on research needs
Dyes optimized for membrane dynamics, lipid trafficking, endocytosis, and exocytosis studies
Support for custom phospholipid headgroup modifications (amine, carboxyl, PEG spacer)
Lipid-labeling strategies designed to retain bilayer stability and fusion properties
Purification via HPLC and verification of dye incorporation efficiency
Suitable for both live-cell and fixed-cell membrane imaging
Customizable dye loading levels for quantitative lipid visualization
High-stability formulations compatible with surfactants, vesicles, and solvent systems

Popular dyes:

Bodipy FL, Bodipy 500/510, DiI, DiO, FITC-lipid analogs, Alexa Fluor

Fluorescent Liposomes & Lipid-Based Nanostructures

Capabilities include:

Preparation of fluorescently labeled liposomes via bilayer insertion, covalent lipid labeling, or encapsulation strategies
Customizable liposome sizes (50–400 nm) via extrusion or microfluidic assembly
Suitable for neutral, cationic, anionic, and PEGylated liposomes
Multi-color liposome labeling for tracking fusion, uptake, biodistribution, and drug release
Compatible with cellular delivery studies, in vivo imaging, and nanomedicine R&D
Encapsulation of fluorescent probes for drug release kinetics or payload tracking
High colloidal stability formulations for serum-containing media
Purification using size-exclusion chromatography for high-purity liposomes
Available surface functionalization (antibody, peptide, aptamer) for targeted delivery
QC including DLS sizing, zeta potential, fluorescence intensity, and encapsulation efficiency

Popular dyes:

DiI, DiO, DiD, Bodipy, Cy5, Cy7, NIR fluorescent dyes

Fluorescent Labeling of Nanoparticles

(Gold, Magnetic, Polymeric)

Capabilities include:

Fluorescent labeling of gold nanoparticles, iron oxide nanoparticles, polymeric nanoparticles, silica NPs, and quantum dots
Surface modification (COOH, NH2, SH, PEG) prior to dye conjugation
Labeling compatible with in vivo imaging, biodistribution tracking, and cellular uptake studies
Stable dye integration that resists photobleaching and surface dissociation
Customizable particle sizes from 10 nm to 500 nm
Multi-color nanoparticle labeling for multiplex tracking
Ligand co-functionalization (antibodies, peptides, aptamers) optional
QC including DLS, zeta potential, fluorescence spectra, and TEM (optional)
Formulations for high colloidal stability in buffers and serum
Suitable for nanomedicine, targeted delivery, and theranostic research

Popular dyes:

FITC, Rhodamine, Cy5, Cy7, Alexa Fluor

Fluorescent Microspheres & Bead-Based Conjugates

Capabilities include:

Fluorescent labeling of polystyrene, silica, and magnetic microspheres
Surface chemistries supported: COOH, NH2, epoxy, aldehyde, streptavidin-coated beads
Custom dye loading for bright, uniform fluorescence in assay development
Multi-color bead production for multiplex diagnostic assays
Covalent attachment of proteins, antibodies, or peptides to fluorescent beads
Support for bead sizes from 100 nm to 20 µm
Ideal for flow cytometry calibration, immunoassays, and cell sorting applications
Fluorescent bead stabilization for long-term storage without signal decay
QC including fluorescence uniformity, particle size distribution, and binding efficiency

Popular dyes:

FITC, PE, Cy3, Cy5, Alexa Fluor, NIR dyes

Fluorescent Labeling of Carbohydrates & Polysaccharides

Capabilities include:

Fluorescent labeling of glycans, polysaccharides, glycoproteins, and carbohydrate derivatives
Hydrazide/aldehyde, amine-reactive, or click-chemistry–based conjugation
Labeling compatible with branched polysaccharides and complex glycan libraries
Ideal for studying glycan–protein interactions and cell-surface sugar dynamics
High-purity conjugates via chromatographic cleanup
Custom linkers to preserve carbohydrate structure and flexibility
Compatibility with lectin binding studies, glycomics, and immune recognition assays
Labeling for in vivo carbohydrate uptake tracking
Spectrally distinct fluorophore options for multi-glycan assays
Stability-optimized formulations for long-term storage and repeated use

Popular dyes:

FITC, TRITC, Alexa Fluor dyes, Bodipy

Fluorescent Labeling of Fatty Acids & Metabolic Tracers

Capabilities include:

Labeling of fatty acids (C6–C20), metabolic tracers, and β-oxidation substrates
Integration of BODIPY, NBD, or custom NIR dyes for metabolic imaging
Suitable for studies of fatty acid uptake, transport, oxidation, and lipid turnover
Custom linker lengths to retain native fatty acid biological behavior
High-purity products (>95%) via HPLC purification
Format optimization for serum-containing conditions or cell-based uptake assays
Labeling strategies designed for lipid droplet imaging and membrane incorporation
Compatible with in vivo metabolic flux analysis
Stability-enhanced formulations to prevent oxidation or degradation
Fluorescence quantification validated for metabolic assays or high-throughput screening

Popular dyes:

Bodipy FL C12, Bodipy C16, Rhodamine-based dyes

Fluorescent Streptavidin Conjugation

Capabilities include:

Fluorescent labeling of streptavidin, neutravidin, and avidin
Conjugation to a broad spectrum of fluorophores (FITC, PE, Cy5, Alexa Fluor series, NIR dyes)
Preserved biotin-binding capacity after labeling through controlled reaction conditions
Ideal for ELISA, flow cytometry, biosensors, and immunoassays
Multi-color streptavidin conjugates for multiplex biotin-binding assays
Compatible with bead-based systems, nanoparticles, and surface-immobilized formats
Purification by SEC to remove free dye and ensure high-purity conjugates
Custom DOL tuning for optimal brightness with minimal steric hindrance
QC includes fluorescence measurement, binding efficiency tests, and SDS-PAGE analysis

Popular dyes:

FITC, PE, APC, Cy3, Cy5, Alexa Fluor

Fluorophores We Offer

To support a wide range of research applications—from cell imaging and flow cytometry to qPCR, nanoparticle tracking, and high-throughput screening—we offer an extensive portfolio of high-performance fluorophores. Our selection includes standard fluorescent dyes, near-infrared probes, and specialized dye chemistries optimized for proteins, antibodies, peptides, nucleic acids, lipids, nanoparticles, and small molecules. Each fluorophore is chosen for its brightness, photostability, spectral clarity, and compatibility with modern detection platforms, enabling precise and reproducible fluorescence-based analysis across diverse scientific workflows.

Fluorophore	Excitation (nm)	Emission (nm)	Applications	Features
FITC (Fluorescein Isothiocyanate)	495	519	Immunofluorescence, flow cytometry, IHC, Western Blot	High brightness, commonly used for antibody labeling
Alexa Fluor 488	495	519	Microscopy, flow cytometry, protein labeling	Bright, photostable, ideal for multicolor panels
Cy3	550	570	FACS, immunohistochemistry, real-time PCR	Bright emission, popular for protein and antibody labeling
Cy5	649	670	DNA hybridization, fluorescence microscopy	Excellent for multiplex assays, low background
Cy7	749	776	In vivo imaging, multiplexing, flow cytometry	Near-IR for deep tissue imaging, photostable
FITC-conjugated Antibodies	495	519	Flow cytometry, cell sorting, immunostaining	Popular for antibody conjugation in diagnostic assays
BODIPY	503–505	510–525	Membrane studies, lipid tracking, high-throughput assays	Lipophilic, ideal for lipid labeling and tracking
DiI (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine)	484	501	Lipid bilayer studies, live-cell tracking	Bright red, excellent for membrane labeling
DiO (3,3'-Dioctadecyloxacarbocyanine Perchlorate)	484	501	Liposome and membrane studies, cellular uptake	Green fluorescence, widely used for lipid studies
Bodipy FL C12	503	513	Fatty acid metabolism, lipid tracking	Hydrophobic, ideal for metabolic studies
Rhodamine B	543	575	Nucleic acid staining, fluorescence microscopy	Strong fluorescence, used for live-cell imaging
TAMRA (Tetramethylrhodamine)	540	565	Protein labeling, fluorescence polarization	Excellent for protein-protein interaction studies
Rox (Rhodamine X)	586	607	PCR, quantitative assays	Excellent for multiplexing and real-time PCR
NIR Dye 800	780	805	In vivo imaging, multiplex assays, animal research	Ideal for deep tissue imaging, low auto-fluorescence
ATTO 488	498	523	Fluorescence microscopy, FRET	High brightness, used for biomolecular interactions

Our Labeling Chemistry & Methods

Achieving reliable and high-performance fluorescent labeling requires selecting the right conjugation strategy for each biomolecule. Our platform integrates a comprehensive suite of bioconjugation chemistries, enabling precise, efficient, and application-specific fluorescent labeling of antibodies, proteins, peptides, nucleic acids, lipids, polysaccharides, nanoparticles, and small molecules. By leveraging NHS ester coupling, maleimide–thiol reactions, click chemistry, enzymatic labeling systems, and advanced site-specific methodologies, we ensure optimal dye attachment, structural integrity, and functional preservation across all project types.

Labeling Method	Chemistry	Common Applications	Advantages
NHS Ester (Amine Labeling)	Uses N-Hydroxysuccinimide (NHS) ester for covalent coupling to primary amines (e.g., lysines) on proteins or peptides.	Protein labeling, Antibody labeling, Peptide conjugation	Simple, robust, fast, and commonly used in protein conjugation
Maleimide (Cysteine Labeling)	Maleimide groups react with thiol groups (-SH) on cysteine residues, forming a stable thioether bond.	Protein modification, Antibody conjugation, Peptide labeling, Labeling cysteine-rich proteins	Highly specific to cysteine residues, minimal side reactions
Click Chemistry (Azide-Alkyne Reaction)	Uses azide and alkyne functional groups that undergo a catalyst-free, strain-promoted reaction.	Site-specific labeling of proteins, Peptide labeling, Fluorescent probe conjugation	Fast, high yield, allows site-specific labeling without disrupting protein function
Carbodiimide Chemistry (EDC/NHS)	Carbodiimides activate carboxyl groups on proteins or other biomolecules to form covalent amide bonds with amines.	Carbohydrate conjugation, Protein conjugation, Peptide labeling	Simple, efficient, mild conditions; commonly used for protein-carbohydrate conjugation
Sortase-Mediated Labeling	Sortase A enzyme catalyzes the attachment of a fluorophore to a sorting tag on proteins.	Site-specific protein labeling, Recombinant protein modification, Peptide tagging	Provides precise, controlled labeling with minimal modification of the protein's function
Enzyme-Catalyzed Labeling	Engineered enzymes selectively bind to fluorophore substrates and incorporate them into proteins.	Tagging proteins, Live-cell imaging, Protein localization studies	High specificity, non-invasive, suitable for live-cell imaging and protein tracking
Photo-Crosslinking Chemistry	Photo-reactive groups (e.g., aryl azides) are incorporated into proteins or peptides and activated under UV light to form covalent bonds with neighboring molecules.	Protein-protein interactions, Receptor-ligand binding studies, Cell signaling	Allows for precise localization of molecular interactions in cells or tissues
Fluorescent Dyes (Nucleophilic Substitution)	Fluorescent dyes (e.g., FAM, TAMRA, Cy5) are conjugated to nucleophilic groups (e.g., amino, thiol, or hydroxyl groups) on biomolecules.	General protein labeling, Nucleic acid labeling, Peptide modification	Provides versatile labeling with a broad range of commercially available fluorophores
Hydrazide/Carbonyl Chemistry	Hydrazides react with carbonyl groups on reducing sugars or aldehydes.	Glycoprotein labeling, Carbohydrate conjugation, Sugar modification	Effective for glycobiology and carbohydrate research
Succinimidyl Ester Chemistry	Succinimidyl esters react with primary amines to form stable amide bonds.	Antibody labeling, Protein conjugation, Peptide modification	Highly reactive, easy-to-use, common for bioconjugation of proteins and peptides

Quality Control & Data Delivered

We provide a comprehensive QC package to ensure the accuracy, purity, and performance of every fluorescently labeled biomolecule. Each project is validated using industry-standard analytical methods, ensuring consistent reproducibility and reliable experimental results.

QC Item	Description / Method	Delivered Data
Purity Analysis	HPLC / SEC / PAGE	Chromatograms, % purity
Dye–to–Molecule Ratio	UV-Vis spectral analysis	DOL / DAR values
Integrity Verification	SDS-PAGE / LC-MS	Gel images, mass confirmation
Fluorescence Characterization	Excitation/emission spectra	Spectral profiles
Binding/Activity Test (optional)	ELISA / functional assay	Activity confirmation
Size Distribution (for particles)	DLS / TEM	Size report, PDI
Stability Assessment	Fluorescence decay / storage stability	Stability data

General Workflow for Fluorescence Labeling Services

Project Assessment & Strategy Design

We evaluate your target molecule, application requirements, and preferred fluorophores. A customized labeling strategy and chemistry selection are designed to ensure optimal performance.

Sample Preparation & Pre-Processing

Incoming samples are inspected, quantified, and buffer-exchanged if necessary. We remove interfering components to ensure efficient, high-yield conjugation.

Fluorescent Labeling Reaction

Labeling is performed using optimized reaction conditions and controlled dye-to-molecule ratios. Both site-specific and conventional labeling strategies are available.

Purification & Removal of Free Dye

Excess dye is removed through SEC, HPLC, or ultrafiltration to achieve high-purity conjugates. Purification is tailored to the biomolecule type.

Quality Control & Data Verification

Each conjugate undergoes purity, DOL/DAR, spectral, and structural integrity analysis. Optional functional assays confirm biological activity when required.

Final Delivery & Technical Support

You receive the labeled product with a complete QC report and handling guidelines. Our team provides follow-up consultation to ensure successful downstream application.

Advantages of Our Fluorescent Labeling Services

High Sensitivity & Specificity

Our fluorescent labeling services deliver high sensitivity and specificity for even the most challenging biological samples, ensuring accurate detection of low-abundance biomolecules in complex matrices.

Versatility Across Biomolecules

We offer fluorescent labeling for a wide range of biomolecules, including proteins, nucleic acids, lipids, small molecules, and nanoparticles, allowing you to address diverse research needs with a single provider.

High-Throughput Capability

With multicolor fluorescence and multiplexing capabilities, our services enable simultaneous detection of multiple targets in a single experiment, making us an ideal partner for high-throughput screening and multicolor imaging.

Real-Time, High-Resolution Data

Benefit from high temporal and spatial resolution in your research, with real-time tracking of protein dynamics, gene expression, and cellular behavior, essential for studying fast biological processes.

Quantitative, Precise Analysis

Our fluorescent labeling technology enables precise and quantitative analysis of biomolecule concentrations and interactions, making it invaluable for accurate assay development and biomarker quantification.

Customized Solutions

We tailor our fluorescent labeling services to meet your unique research goals, including site-specific labeling, custom fluorophore selection, and highly specific conjugation methods that suit your exact requirements.

Applications of Fluorescent Labeling Technology

Imaging

Visualize cellular structures, organelles, and protein localization using fluorescent probes.
Track live-cell dynamics such as migration, division, apoptosis, and intracellular transport.
Map spatial distribution of biomolecules with high-resolution fluorescence microscopy.
Perform multi-color imaging to study complex biological interactions in real time.

Flow Cytometry

Identify and quantify specific cell populations using fluorescent antibody panels.
Perform multi-parameter immunophenotyping with minimal spectral overlap.
Analyze cell viability, activation markers, and intracellular proteins with high sensitivity.
Enable high-throughput cell sorting based on fluorescence intensity and biomarker expression.

Diagnostics

Develop fluorescence-based assays for biomarker detection.
Enhance qPCR and molecular diagnostic platforms with fluorescent probes and dNTPs.
Improve sensitivity and specificity in immunoassays using labeled detection antibodies.
Support point-of-care and high-throughput diagnostic systems with robust fluorescence readouts.

Molecular Biology

Track DNA, RNA, and siRNA delivery, localization, and intracellular trafficking.
Monitor nucleic acid hybridization efficiency using fluorescent probes.
Study gene expression, replication, repair, and transcription dynamics with labeled nucleotides.
Enable live-cell nucleic acid imaging with high-stability fluorophore-tagged constructs.

Drug Discovery

Perform binding assays using fluorescently labeled small molecules, peptides, or receptors.
Accelerate high-throughput screening with robust fluorescence-based readouts.
Track compound uptake, biodistribution, and cellular trafficking in drug evaluation.
Develop fluorescent probes for target validation and mechanism-of-action studies.

Nanotechnology

Track nanoparticle uptake, distribution, and intracellular pathways using fluorescent labeling.
Visualize nanocarrier behavior in live cells and animal models.
Evaluate targeted delivery efficiency with fluorescence-tagged nanoparticles or liposomes.
Support nanomedicine development through high-resolution fluorescence imaging.

Lipid & Membrane Studies

Analyze membrane fluidity, domain organization, and lipid raft dynamics.
Trace lipid metabolism and transport using fluorescent fatty acids and phospholipids.
Visualize vesicle formation, fusion, and endocytic pathways.
Investigate drug–membrane interactions using fluorescence-based assays.

Glycobiology

Study glycan–protein interactions with fluorescently labeled carbohydrates.
Visualize cell-surface glycan distribution in live or fixed samples.
Track polysaccharide uptake, metabolism, and cellular processing.
Develop fluorescent glycan probes for glycomics and immune recognition studies.

Case Study

Case Study 1

The process of fluorescence labeling DNAs is critical yet the existing techniques tend to be costly and labor-intensive. We introduce a novel, efficient, and affordable method for fluorescence labeling of oligonucleotides using base excision trapping (BETr). This method utilizes deaminated DNA bases to identify label positions and aminooxy-substituted rotor dyes to highlight AP sites, leading to high emission intensities. Importantly, BETr can work in conjunction with DNA synthesis by polymerases, allowing for the integration of multiple uracil into an amplicon and in situ BETr labeling without any need for washing. Furthermore, BETr permits high labeling density of dsDNA such as genomic DNA in a single tube through nick translation. By using two different deaminated bases, two-color site-specific labeling is achieved. This methodology also utilizes a multi-labeled DNA construct as a luminescent tag, an added advantage demonstrated by linking to an antibody for protein imaging. The technique also leverages the double-strand selectivity of a repair enzyme in accurately detecting the presence of a target DNA or RNA in a mixed sample with isothermal turnover and single nucleotide specificity. Hence, BETr offers a highly adaptable and easy way for general fluorescence labeling of DNAs.

Fig.2 In situ DNA synthesis and BETr labeling. (Jun, Y.W., 2022)

Case Study 2

The recent notable rise in the usage of deep eutectic solvents (DESs) offers novel methodologies for sample collection and preparation. However, utilizing these new solvents poses difficulties for subsequent analyses since standard analytical techniques, like fluorescence labeling, may be incompatible. This study investigates the efficacy of three conventional fluorescent labels in derivatizing amino acids in prevalent DESs, comprised of choline chloride and ethylene glycol. We found that the distinctive solvent attributes of the DESs permit two fluorophores, fluorescein isothiocyanate and 5-carboxyfluorescein succinimidyl ester, to successfully label amino acids. Conclusions indicate the feasibility of effectively labeling both D- and L-amino acids at concentrations as low as 4 μM in solution. Following this process, capillary electrophoretic separation is able to detect as low as 50 nM, without the need to eliminate any DES. This is the first time a total fluorescent labeling reaction has been carried out in a DES, followed by capillary electrophoretic separation of the analytes.

Fig.3 Representative electropherograms of various amino acids simultaneously reacted in 5 mM KOH ethaline at (1) Leu, (2) L-His, (3) L-Ser, (4) L-Ala, (5) Gly, and (6) L-Glu, all at 25 μM amino acid with FITC. (2. Torres, J., 2022)

Frequently Asked Questions (FAQ)

What is fluorescence labeling?

Fluorescence labeling is a technique used in biological and medical research to tag or highlight specific proteins, cells, or other molecules of interest. This is achieved by attaching fluorescent dyes or proteins to the target molecules, which emits light of a longer wavelength when excited by light of a shorter.

How does fluorescence labeling work?

The process of fluorescence labeling involves attaching fluorescent dyes or proteins to the target molecules. Once these molecules are subjected to light of a certain wavelength, they absorb the light energy and re-emit it as light of a longer wavelength, producing a glow that can be detected and analyzed.

What is fluorescence labeling of DNA?

Fluorescence labeling of DNA is a technique used in molecular biology where fluorescent dyes are attached to DNA molecules. These labeled DNA fragments can then be visualized under a microscope or in an analytical instrument through the generation of a fluorescent signal. This method is commonly used in various DNA tests and experiments including gene mapping, DNA sequencing, DNA microarrays, and studies involving gene expression and regulation.

How does DNA fluorescence work?

It works on the principle that certain chemicals, known as fluorescent dyes, can intercalate (slot between) the bases (A, T, C, G) of DNA. When these dyes are exposed to a specific wavelength of light, they absorb the energy and enter into an excited state. After a short period, they drop back down to their normal energy state and give off a photon - this is the fluorescence that can be detected.

What are the advantages of using fluorescence labeling?

This technique allows for the visualization and analysis of specific biological substances in a much clearer and more detailed manner than traditional methods. It aids in studying cellular processes, protein-protein interactions and the distribution of molecules within cells. It is highly sensitive, precise, and can be used in live cells and organisms.

In what fields can fluorescence labeling be used?

Fluorescence labeling can be utilized in many biological and medical research fields. This encompasses cell biology, microbiology, immunology, genetics, biochemistry, and more. They are frequently used in diagnostic assays, drug discovery and development, as well as in medical imaging procedures.

Are there any risks or side effects of fluorescence labeling?

The main challenge of fluorescence labeling is to introduce the fluorescent label without interfering with the function of the molecule or system of interest. Some fluorescent tags can induce harmful effects when exposed to light, potentially causing cellular damage. Therefore, careful calibration and handling procedures are crucial in the use of this technique.

How much does fluorescence labeling service cost?

The cost of fluorescence labeling service can vary greatly depending on the type and amount of material to be labeled, the complexity of the procedure, and the specific fluorescent label used. Please contact us directly for a more specific and accurate quotation.

What Our Research Clients Say

"Their expertise in fluorophore selection and conjugation chemistry saved us substantial optimization time. The fluorescent nucleotides integrated perfectly into our workflows."

— Dr. M., Head of Technology, UK-based Biotechnology Company

"Their custom fluorescent oligo labeling performed flawlessly in our diagnostic assay development. Outstanding brightness with minimal background noise."

— Ms. Y., Senior Molecular Scientist, European Diagnostics Manufacturer

"The fluorescent liposome and nanoparticle conjugates significantly improved our in vivo imaging performance. Excellent reproducibility across batches."

— Dr. Z., Principal Researcher, Asia-Pacific Nanomedicine Startup

Custom Fluorescent Labeling Solutions for Every Research Need

No matter what your research requires—whether it's fluorescent nanoparticles, RNA studies, or enzymatic assays—we provide customized fluorescent labeling that fits your specific needs. Our team of experts is here to guide you through every step of the process, from dye selection to conjugation strategy, ensuring that your research is both successful and reproducible. Reach out to us for a quote or consultation, and let us help you with the ideal fluorescent labeling solution tailored to your research needs.