Fluorescence Labeling of Enzyme

* Please be kindly noted that our services can only be used for research to organizations or companies and not intended for any clinical or individuals.

As a leading service provider in the field of drug discovery and research, BOC Sciences is fully competent and dedicated to serving one-stop molecule labeling services which has be used in various detection and assay system. Our unique and unparalleled expertise in bioconjugated chemistry provides you with the highest quality fluorescence labeling enzyme services in the industry.

Introduction

In molecular biology and biotechnology, fluorescent labels (also known as fluorescent probes) are chemically linked molecules that help detect biomolecules, such as proteins, antibodies or amino acids. In general, fluorescent labeling, uses a fluorophore of a reactive derivative called a fluorescent molecule. The fluorophore selectively binds to a specific region or functional group of the target molecule and can be connected chemically or biologically. Various labeling techniques, such as enzyme markers, protein markers and gene markers, are widely used. Ethidium bromide, fluorescein and green fluorescent protein are common labels. The most commonly used marker molecules are antibodies, proteins, enzymes and peptides, which are then used as specific probes for specific targets.

Application

In the enzyme labeling, the DNA of gene and fluorescent protein were first used to form the DNA construct. After transcription, hybrid RNA fluorescence was formed. The object of interest is connected to an enzyme that recognizes this heterozygous DNA. Fluorescein or biotin is usually used as a fluorescent group. The lipase was labeled with fluorescence so as to establish the relationship among fluorescence spectrum, enzyme activity and conformational change of lipase.

Our Advantages

  • Unique and unparalleled expertise
  • Dedicated technical account manager
  • Label scale can be optimized according to customer requirements
  • Strict quality assurance and quality control
  • Many different types of studies including spectroscopic (MALDI-TOF, ESI, LC-MS Fluorescence), electrophoresis, immunochemical biochemical, enzymatically analysis
  • Data analysis, detailed report with results and discussion

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