Fluorescence Labeling of Enzyme
BOC Sciences, as a leading service provider in the field of drug discovery and research, is fully capable and committed to providing fluorescent molecular labeling services that can be used in a variety of detection and assay systems. Our unique and unparalleled expertise in bioconjugation chemistry provides you with the highest quality fluorescent labeling of enzymes services in the industry.
What is Fluorescent Labeling of Enzymes?
Fluorescent labeling of enzymes refers to the property of imparting a fluorescent signal to an enzyme by binding a fluorescent dye to the enzyme. This labeling method introduces fluorescence into the enzymes, enabling fluorescence detection and visualization of the enzymes. In the fluorescent labeling of enzymes, the selection of suitable fluorescent dyes and appropriate coupling methods are the key steps. Common fluorescent dyes include fluorescein, rhodamine, fluorescent yellow, etc., which have fluorescence spectra emitting specific wavelengths.
Fluorescent Labeling Enzyme Services
Fluorescent labeling experiment design
We provide suggestions for experimental design and optimization based on the customer's experimental needs and the characteristics of the target molecule. This may involve the selection of fluorescent dyes and enzymes, optimization of reaction conditions, determination of reagent concentration, etc.
Fluorescent labeling experiments
According to customer requirements, BOC Sciences performs fluorescent labeling experiments and provides relevant experimental data and results. This part includes the evaluation of fluorescent labeling efficiency, specificity and stability of target molecules.
Customized fluorescent labeling enzymes
If large quantities of purified fluorescent marker enzymes are required, we can purify and customize the enzymes. This includes expression and purification of the target enzyme from an appropriate expression system, followed by fluorescent labeling.
Technical support and consulting
We can provide technical support and consultation on fluorescent labeling enzymes to help customers solve problems encountered in their experiments and provide experimental protocols and suggestions.
Steps for Preparing Fluorescent Labeling Enzyme
- Obtaining the target enzyme: First, it is necessary to obtain the purified target enzyme. This can be done either by extracting the target enzyme from biological samples (e.g. cell extracts or tissues) or by expressing and purifying the target enzyme through genetic engineering techniques.
- Preparation of the fluorescent dye: Selection and use of a consistent fluorescent dye.
- Coupling the dye to the enzyme: Coupling the fluorescent dye to the target enzyme. This can be achieved by chemical cross-linking reactions or specific coupling reagents. The coupling reagent can react with specific functional groups on the enzyme or dye to covalently bind the dye to the enzyme molecule.
- Purification and isolation: Purification and isolation of the fluorescently labeled enzyme to remove uncoupled fluorescent dye and other impurities.
- Identification and validation: Identification and validation of the prepared fluorescent labeling enzyme. Techniques such as enzyme activity assays, SDS-PAGE gel electrophoresis, mass spectrometry and fluorescence spectroscopy can be used to confirm the presence and correctness of the fluorescently labeled enzyme.
Our Advantages
- Unique and unparalleled expertise
- Dedicated technical account manager
- Label scale can be optimized according to customer requirements
- Strict quality assurance and quality control
- Many different types of studies including spectroscopic (MALDI-TOF, ESI, LC-MS Fluorescence), electrophoresis, immunochemical biochemical, enzymatically analysis
- Data analysis, detailed report with results and discussion
Project Workflow
Frequently Asked Questions (FAQ)
Fluorescence labeling of enzymes involves attaching a fluorescent dye to the enzyme molecule. This allows researchers to track enzyme activity, localization, and interaction with substrates or inhibitors through fluorescence-based detection methods.
We ensure that the fluorescent labeling process does not significantly affect enzyme activity by optimizing the conjugation conditions. In most cases, the labeling is carefully controlled to preserve the enzyme's functionality, but each enzyme's sensitivity to labeling may vary.
Fluorescently labeled enzymes allow for live-cell imaging, enabling researchers to track the enzyme's localization and movement within cells in real time. This is especially useful for studying enzyme trafficking, organelle targeting, and cellular response to environmental changes.
Yes, enzymes can be labeled with different fluorophores to enable multi-parameter assays. This allows researchers to track multiple enzyme activities or interactions simultaneously, providing a more comprehensive understanding of complex biochemical pathways.
The choice of labeling strategy depends on the enzyme's characteristics, such as size, function, and interaction with substrates. For example, small enzymes may benefit from direct labeling, while larger enzymes or those with specific binding sites may require site-specific labeling techniques to preserve activity and specificity.
