Magnetic Beads labeled Protein
Magnetic beads labeled proteins are commonly used in a variety of biological and biomedical applications including diagnostics, immunoassays and protein purification. These beads combine the magnetic properties of the beads with the specific binding ability of the labeled protein, allowing for efficient isolation, detection, and manipulation of the target molecule. BOC Sciences has many years of experience in nanoparticles & beads biocoupling, and we design, evaluate, and select the most appropriate coupling technology based on efficacy and application to efficiently and cost-effectively deliver the high-quality bioconjugates. Our services will assist our customers in the screening and optimization of magnetic beads at different stages of label development.
Protein Magnetic Beads
Magnetic beads are superparamagnetic and can realize rapid separation of bound and unbound proteins under the action of magnetic field, simplifying the operation and shortening the reaction time. Magnetic beads have significant advantages as the carrier of the whole immune reaction and signal collection: (1) Magnetic beads have a large specific surface area and can bind more protein molecules, which can improve the detection range. (2) The chemical groups on the surface of magnetic beads form covalent coupling with proteins, which is stronger and more stable than physical adsorption.
Magnetic Bead Labeling Protein Services
BOC Sciences offers a comprehensive range of magnetic bead labeling development services, including magnetic bead modification, protein conjugation and more. With unique expertise in chemical synthesis and biochemistry, we are confident in providing one-stop bioconjugation services to meet your specific needs.
Surface Modification of Magnetic Beads Services
BOC Sciences provides surface modification services for magnetic beads by selecting magnetic beads of appropriate size and magnetic properties and washing them with appropriate washing buffers to remove surface impurities. Afterwards, the magnetic beads are incubated with a chemical cross-linking agent (e.g. EDC/NHS) to activate the reactive functional groups on the surface of the magnetic beads. Finally, the target binding molecules are reacted with the activated magnetic beads to form covalent binding.
Protein Binding Services
Protein samples to be labeled are incubated with modified magnetic beads under appropriate conditions (e.g., temperature, pH, etc.) to allow specific binding of the target protein to the binding molecules on the surface of the magnetic beads.
Applications of Magnetic Beads Labeled Protein
Drug Screening and Target Recognition
Magnetic bead-labeled proteins can be used for drug screening and target identification. By binding drug candidates to target proteins on magnetic beads, the affinity and specificity between the drug and the target protein can be assessed, thus helping to screen compounds for potential therapeutic effects.
Cell Therapy
Magnetic bead labeling of proteins has applications in cell therapy. By labeling specific proteins onto magnetic beads, therapeutically relevant cells or subpopulations of cells can be selectively labeled and enriched. These labeled cells can be used in areas such as cell transplantation, gene therapy, and stem cell research to facilitate the effectiveness of cell therapy.
Phage Display Technology
After binding the magnetic bead-labeled protein specifically bound to the target molecule to the phage displayed protein, the phage particles displayed by the target protein can be separated by magnetic field. This allows easy access to target protein-exhibiting phages for subsequent analysis, such as structural studies, affinity assays, and enzyme activity assays.
Project Workflow
Frequently Asked Questions (FAQ)
Magnetic beads are used to bind and isolate proteins, enabling easier separation and detection in assays. They facilitate rapid and efficient purification or enrichment of target proteins.
Magnetic beads simplify protein purification by binding proteins with specific functional groups, allowing for easy separation from unbound materials when exposed to a magnetic field.
Magnetic beads are modified with chemical cross-linkers like EDC/NHS, activating surface groups to covalently bind proteins, ensuring strong and stable interactions for reliable results.
The labeling process typically takes a few hours, depending on the protein and magnetic bead characteristics, as well as the binding and washing steps.
