Gold Nanoparticles labeled DNA
Gold nanoparticles labeled DNA technology offered by BOC Sciences has a wide range of applications in biological research and biomedical applications, such as for, biosensors, cell imaging and localization. This technology enables the visualization, quantitative analysis and detection of DNA, opening up new possibilities for scientific research and applications.
Fig.1 An illustration of the process to prepare ss-DNA dual labeled gold nanoparticle. (Qiao et al., 2008)
DNA Gold Nanoparticles
DNA gold nanoparticles are composite materials that combine gold nanoparticles with DNA molecules. In this composite, gold nanoparticles are used as labeling agents and DNA molecules are used as carriers or targeting molecules. Gold nanoparticles labeled DNA is an experimental technique based on the unique properties of gold nanoparticles combined with the high selectivity and specificity of DNA. Thus, the combination of gold nanoparticles and DNA is used to detect bases, single strands and oligonucleotides of DNA. The use of nanoparticles as labels for the detection of DNA is an interesting alternative to standard fluorescence techniques. The method has simple detection equipment, high stability and low cost.
Gold Nanoparticles Labeled DNA Services
BOC Sciences is a chemical and biotechnology service provider that offers a broad range of nanoparticles & beads conjugation services. We provide:
- Gold nanoparticles labeled DNA for tumor diagnosis
- Bioprobe-related gold nanoparticles labeled DNA
- Biomarker-related gold nanoparticles labeled DNA
- Gold nanoparticles labeled DNA for optical and electrochemical sensors
- Gold nanoparticles labeled DNA as a drug carrier
The gold nanoparticles labeled DNA services include:
- Synthesis of DNA Functionalized Gold Nanoparticles
BOC Sciences can synthesize DNA with specific sequences and prepare DNA functionalized gold nanoparticles by labeling the DNA with gold nanoparticles according to the needs of customers. During the labeling process, the gold nanoparticles are usually bonded to the DNA sequences by physical or chemical methods. By exploiting the affinity of DNA, a stable binding is formed by pairing the DNA sequence with an affinity ligand (e.g., a sulfide or amine group) on the surface of the gold nanoparticles. This binding process can be verified and characterized by methods such as UV-Vis absorption spectroscopy and electron microscopy.
- Characterization and Analysis of Gold Nanoparticles Labeled with DNA
We provide services to characterize and analyze labeled DNA samples, including nucleic acid concentration determination, purity analysis, and characterization of gold nanoparticles (e.g., morphology, size, surface charge, etc.). Through the above analytical tests, the labeling effect of gold nanoparticles and the quality of DNA can be verified. Common characterization methods include UV-Vis absorption spectroscopy, dynamic light scattering, transmission electron microscopy, etc.
- Biological Activity Studies
Labeled DNA samples can be applied in various biological studies, biosensors or drug delivery. We offer bioactivity studies on labeled DNA, such as evaluation of transfection efficiency and cytotoxicity of DNA in cells.
Applications of Gold Nanoparticles Labeled DNA
- Biosensors
Detection and quantification of target molecules (e.g. proteins, pathogens or tumor markers) can be achieved by combining specific DNA sequences with gold nanoparticles. When the target molecule matches the DNA sequence, the aggregation state of the gold nanoparticles changes and can be detected optically.
- Cellular imaging and localization
By introducing gold nanoparticle-labeled DNA into cells, the position and distribution of gold nanoparticles can be observed through microscopy, so as to study DNA transfection efficiency, intracellular localization, and gene expression.
- DNA nanotechnology research
By interacting gold nanoparticles with DNA molecules, DNA nanostructures with specific shapes and structures can be constructed. Such nanostructures can be used for research and applications in gene delivery, nano-circuits, optoelectronics, and other fields.
Project Workflow
Reference
- Qiao F Y, et al. Antibody and DNA dual-labeled gold nanoparticles: Stability and reactivity[J]. Applied surface science, 2008, 254(10): 2941-2946.