Improved Cellular Uptake Optimized Therapeutic Delivery Enhanced Functionality
We offer expert oligonucleotide conjugation services that ensure precise molecular targeting and enhanced stability for your nucleic acid-based projects. Our advanced conjugation techniques improve cellular uptake and binding affinity, providing tailored solutions for research, diagnostics, and therapeutic applications. Trust us for efficient, custom-designed oligonucleotide conjugates that boost the effectiveness of your biomolecular innovations.
Fig. 1 Oligonucleotide conjugatesOligonucleotides are short DNA or RNA molecules that have a wide range of applications in genetic testing, research, and forensics. They are typically composed of 13 to 25 nucleotides; this small size allows for precise binding to specific sequences in the DNA or RNA of an organism, making them useful in techniques such as PCR, molecular cloning, and DNA sequencing. The four types of nitrogenous bases in DNA oligonucleotides are adenine (A), thymine (T), guanine (G), and cytosine (C), while RNA oligonucleotides include uracil (U) instead of thymine. The specific sequence of these bases in an oligonucleotide determines its function and interaction with other molecules.
Oligonucleotide conjugation is the process of chemically linking oligonucleotides to functional molecules—such as peptides, proteins, antibodies, fluorophores, lipids, or nanoparticles—to enhance their stability, specificity, and delivery efficiency. This versatile biotechnology enables targeted drug delivery, improved diagnostic sensitivity, and advanced therapeutic development, making it a critical tool in molecular biology, genomics, and precision medicine.
Oligonucleotides, such as DNA, RNA, and PNA (peptide nucleic acid), can easily bind to their complementary strands with high specificity, allowing for precise targeting in biological applications.
Oligonucleotides can be conjugated with various other compounds, including proteins, peptides, fluorescent dyes, and nanoparticles, providing a great deal of versatility in their use.
The attachment of fluorescent dyes or other labels to oligonucleotides allows for the visualization of molecular structures and processes in real time, aiding in the understanding of biological systems.
Conjugation can enhance the stability of oligonucleotides in biological systems, increasing their resistance to degradation by nucleases.
Conjugation with specific targeting ligands or nanomaterials can improve the delivery efficiency of oligonucleotides to target cells or tissues.
Custom Conjugation Strategy Design & ConsultationWe provide tailored oligonucleotide conjugation strategies for various applications, including siRNA, antisense oligonucleotides (ASOs), aptamers, and molecular probes.
Oligonucleotide Modification & SynthesisWe offer high-quality synthesis of modified oligonucleotides with a wide range of functional groups and chemical backbones.
Conjugation Reaction ExecutionOur lab ensures precise and reproducible conjugation under controlled conditions.
Purification & Analytical CharacterizationWe use advanced purification and analytical techniques to ensure product quality and consistency.
Purification: HPLC, PAGE, gel electrophoresis, size-exclusion chromatography (SEC)
Analysis:
Quality Control & Documentation SupportComprehensive quality assurance ensures traceability and reliability.
Custom Application DevelopmentWe offer end-to-end development solutions tailored to your research or clinical goals:
We support conjugation with a broad spectrum of functional molecules to meet different research and therapeutic needs:
Precision enzyme labeling of oligonucleotides for enhanced detection and versatile biochemical applications.
High-quality biotin-labeled oligonucleotides designed for efficient affinity purification and molecular targeting.
Bright and stable fluorescence labeling of oligonucleotides to support sensitive imaging and diagnostics.
Innovative carbohydrate-oligonucleotide conjugation for improved cellular interaction and targeted delivery.
Gold nanoparticle-labeled oligonucleotides providing superior signal amplification for biosensing and therapeutics.
PEG-conjugated oligonucleotides enhancing stability and circulation time for advanced therapeutic development.
Custom antibody-oligonucleotide conjugation enabling precise targeting and novel diagnostic applications.
Peptide-oligonucleotide conjugates designed for efficient cellular delivery and functional modulation.
Protein-oligonucleotide conjugation services tailored for targeted therapy and molecular research.
Cholesterol-conjugated oligonucleotides to improve membrane affinity and enhance cellular uptake.
Advanced oligonucleotide-loaded lipid nanoparticles for effective gene delivery and therapeutic applications.
Small molecule-oligonucleotide conjugation offering versatile targeting and functional enhancements.
Efficient oligonucleotide liposome conjugation for improved stability and targeted drug delivery.
BOC Sciences will employ the appropriate conjugation methods according to the customer's needs to attach the desired molecule to the oligonucleotide without disrupting its structure and function.
Common reactive groups used for chemical conjugation include -NH2, -SH, and -COOH.
Common enzymes used for oligonucleotide conjugation include terminal transferases, ligases, and transglutaminases.
It is a powerful technique used in the efficient and automated production of oligonucleotides, allowing the attachment of molecules to oligonucleotides in a controlled manner.
Commonly used click chemistry reaction for oligonucleotide conjugation include CuAAC, SPAAC.
The photoreactive groups are introduced to the oligonucleotide and the molecule to be attached.
Biotin-streptavidin-based conjugation relies on the strong and specific interaction between biotin and streptavidin.
Our team of scientists has years of experience in the field of Oligonucleotide Bioconjugation, enabling us to provide high-quality services in this specialized area.
We use state-of-the-art technology and techniques that allow us to be accurate and efficient in our service delivery.
We offer personalized solutions to our clients to meet their unique requirements in Oligonucleotide Bioconjugation.
We adhere to strict quality control procedures to ensure we deliver reliable and credible services to our clients.
We provide our top-notch services at competitive prices, ensuring value for money.
We respect our client's proprietary information and ensure the utmost confidentiality of any project-related data.
We are dedicated to continuous research and development to stay ahead of advancements in the field and provide the latest solutions.
We offer our services globally, being able to cater to clients across different geographic locations and time zones.
By coupling therapeutic agents with oligonucleotides, researchers can achieve highly specific drug delivery to specific cells or tissues, minimizing off-target effects and reducing systemic toxicity. Additionally, the ability to modify oligonucleotides allows for the fine-tuning of drug release kinetics.
Modified oligonucleotides can be designed to target disease-causing genetic mutations, inhibit the expression of harmful proteins, or correct genetic defects.
Oligonucleotide conjugates have been instrumental in enhancing the sensitivity and specificity of diagnostic assays. By coupling oligonucleotides with fluorescent or radioactive labels, researchers can create highly sensitive probes for detecting specific DNA or RNA sequences. Furthermore, the modified oligonucleotides have been served as ideal tools for a range of diagnostic applications, including PCR-based assays, in situ hybridization, and nucleic acid microarrays.
Oligonucleotides can be attached to various nanoparticles, such as liposomes, polymeric nanoparticles, or gold nanoparticles, enabling targeted delivery and controlled release of therapeutic oligonucleotides. These nanoscale delivery systems offer unique advantages, such as prolonged circulation time, enhanced cellular uptake, and reduced immunogenicity.
Oligonucleotide bioconjugates can function as immune stimulants or antisense molecules, making them valuable tools in the development of new immunotherapies.
In lab research, bioconjugated oligonucleotides can be used for various purposes, such as studying gene expression, investigating protein-DNA interactions, or tracking the location and movement of specific molecules within cells.
Fluorescent and nanoparticle-conjugated oligonucleotides allow for high-resolution imaging of molecular targets in live cells or tissues. These tools are invaluable in tracking gene expression, studying cell behavior, and discovering new biomarkers for precision medicine and translational research.
Conjugated oligonucleotides such as antisense oligos, siRNA, or aptamers can be chemically modified to improve nuclease resistance and bioavailability. This supports genome-wide screening, gene knockdown experiments, and therapeutic gene silencing with greater efficiency and stability.
Gene therapy has offered promise for treating gene-related diseases. Synthesis and modification simplicity, physiological safety and stability are some of the merits that have contributed to the success of LONs in the delivery of gene molecules for the induction or knockdown of specific gene expression. This research reported two methods for conjugating specific RNA strands with hydrophobic chains, allowing the formation of RNA-amphiphiles and subsequent efficient gene-silencing capability. By employing dibenzocyclooctyne group (DBCO)-labeled DNA-amphiphile as a template, azide-modified RNA was directly attached to the hydrophobic chain via 'click-chemistry' and the hybridized DNA could then be removed by adding DNase I. This interesting method provided a novel solution to the challenge of linking RNA strands with hydrophobic molecules, and the outcome has improved the efficacy of RNA therapeutics.
Fig.2 Gene-silencing using RNA amphiphiles. (Dore, M. D., 2018)
In this study, lipid nanoparticles (LNPs) loaded with siRNA targeting Polo-Like Kinase 1 (PLK1) protein, present in the triple negative breast cancer cell line (MDA-MB-231), have been modified with antibodies to target tumors. Biodistribution studies of labeled siRNA-LNPs demonstrated that antibody modified LNP (antibody against heparin-binding EGF-like growth factor, αHB-EGF) effectively delivered siRNA to tumor tissue in mice. Interestingly, the PLK1 protein expression was inhibited after intravenous injection of the LNPs and tumor growth was decreased. These results indicate that antibody modified LNPs loaded with siRNA are a promising therapeutic approach for breast cancer.
Fig.3 Anticancer effect of αHB-EGF LNP-siPLK1 in vivo. (T Okamoto, A., 2018)
We use several types of linkers in our oligonucleotide bioconjugations. These include disulfide linkers, thioether linkers, and maleimide linkers among others.
Yes, you may certainly provide your own oligonucleotide for bioconjugation. However, we will first conduct a thorough evaluation to ensure that it meets our quality standards.
The time frame can vary depending on the complexity of the project. Generally, it takes around 2-3 weeks. We will provide an estimated timeline after we carefully evaluate the project requirements.
Yes, any service project we undertake is strictly confidential, and you will retain all intellectual property rights related to your bioconjugated molecule.
Absolutely. We offer custom bioconjugation services tailored to the specific needs of your research project or application.
Your conjugated oligonucleotides will be delivered lyophilized, protected from light, and under conditions that ensure the maintenance of product stability. We recommend storing the product at -20 °C for long-term use.
