Fluorescence Labeling of Proteins

Fluorescently labeled proteins are fluorescently labeled by binding a fluorescent dye to the target protein. Fluorescent labeling methods include chemical labeling and genetically engineered labeling, which allows the target protein to have fluorescent properties so that it can be visualized and tracked by equipment such as fluorescent microscopes. BOC Sciences specializes in fluorescent labeling of proteins, offering a wide range of options to meet specific research needs. The customization process includes selecting the appropriate fluorescent probe, determining the optimal labeling site on the protein, and ensuring the stability and functionality of the labeled protein.

Fig.1 Fluorescent Labeling of Proteins and Polymers Using Dithiomaleimides. (Robin et al., 2013)

Fluorescent Labeled Protein Services

BOC Sciences, a dedicated fluorescent labeling service provider, offers high-quality protein fluorescent labeling services. We can utilize our professional laboratory platforms (e.g., fluorescent labeling platform, protein screening platform, and flow cytometry platform, etc.) and abundant fluorescent labeling technologies to label various proteins for our clients. In addition, we can also provide the characterization of fluorescently labeled proteins, stability testing and fluorescence effect evaluation services.

The steps of fluorescent labeling of proteins include:

Fluorescent dye selection

BOC Sciences can select fluorescent dyes for different applications. Common dyes include fluorescein and rhodamine derivatives. The choice of fluorescent dyes depends on the experimental requirements, compatibility with biological systems, and the desired sensitivity of the assay.

Labeling site determination

We employ advanced techniques, such as site-specific labeling using genetic engineering methods or enzymatic splicing, to ensure that the fluorescent probe attaches precisely to the desired location on the protein.

Stability and functionality considerations

With extensive experience and expertise, BOC Sciences employs optimized labeling protocols to minimize protein denaturation or aggregation. Careful selection of labeling conditions ensures that labeled proteins retain their natural properties, enabling accurate representation of their behavior in biological systems.

Fluorescent Protein Labeling Services Contents

• Fluorescent labeling of transporter proteins - including hemoglobin, glucose transporter proteins, and others. Labeling such proteins can be used to study the localization and interaction of transcription factors and other regulatory proteins in the nucleus.
• Fluorescent labeling of structural proteins - including fluorescent labeling of collagen, actin, etc.
• Fluorescent labeling of receptor proteins - including G protein-coupled receptors, tyrosine kinase receptors, etc.
• Fluorescent labeling of cytoskeletal proteins: including labeling of actin, microtubule proteins, etc. Fluorescent labeling of such proteins can be used to study the distribution and dynamic changes of cytoskeletal proteins.

In addition to the above types of proteins, we can also fluorescently label fibronectin, RNA-binding proteins, etc. We can customize a solution for your project.

Applications of Fluorescently Labeled Proteins

• Immunocytochemistry
• Protein dynamics in living cells
• Fluorescence in situ hybridization (FISH)
• Cellular tracers
• Receptor labeling
• Fluorescence Spectroscopy

Our Advantages

• Different strategies are used to label proteins with dyes
• Optional services: Amino acid Analysis and Sequencing
• Excitation and emission spectra for each product
• Analytical HPLC and MS analyses are performed in every development cycle.
• Deep knowledge and rich experience in biomaterial modification and conjugation
• The minimum dosage is only 1 mg.
• Standard packaging can be scaled to 10 mg.
• Simplified multi-signal analysis (for example, co-location research)

Frequently Asked Questions (FAQ)

How can I quantify the efficiency of protein fluorescence labeling?

We offer HPLC and UV-Vis spectrophotometry services to measure labeling efficiency and confirm that the labeling reaction was successful. We can also provide fluorescence intensity measurements to help assess the effectiveness of the labeling.

Does fluorescent labeling affect the protein's function or structure?

We optimize our labeling protocols to minimize interference with the protein's structure and function. We also provide functional assays to verify that the labeled protein retains its biological activity after labeling.

Can I label multiple proteins with different fluorophores for simultaneous detection?

Yes, we offer multi-color labeling services, enabling you to label different proteins with distinct fluorophores for simultaneous detection. Our team ensures that the fluorophores selected have minimal spectral overlap for accurate, multiplexed measurements.

How do I know if my protein labeling is interfering with the protein's interaction with its target?

To ensure labeling does not interfere with protein interactions, we recommend performing binding assays, such as ELISA or co-immunoprecipitation. These tests help confirm that the labeled protein maintains its ability to interact with its target or other biomolecules.