As a leading CRO focused on bioconjugation, BOC Sciences provides custom services for the introduction of biotin molecules onto glycosphingolipids. Our biotinylated glycosphingolipids are ideal for use in sphingolipid research and drug discovery. We are fully competent and dedicated to serving one-stop biotinylation services, allowing for the detection, isolation, and characterization of glycosphingolipids in biological systems.
Glycosphingolipid is a complex lipid consisting of a hydrophobic sphingosine or ceramide backbone, a carbohydrate moiety (monosaccharide or oligosaccharide), and a fatty acid. Glycosphingolipids are an essential component of cell membranes and play crucial roles in cell recognition, cell signaling, and modulation of membrane properties. In addition, glycosphingolipids have been implicated in several diseases and pathological conditions.
The biotin modification of glycosphingolipids allows for their selective labeling and detection in experimental settings. Biotin is a small molecule that has a high affinity for the protein avidin or streptavidin, forming a stable complex. By introducing biotin molecules, glycosphingolipids can be easily tracked and analyzed through affinity chromatography, ELISA, or fluorescent staining methods.
Biotinylated glycosphingolipids are commonly used as probes to study glycosphingolipid metabolism, trafficking, and cellular localization. They can be incorporated into artificial membranes or liposomes to mimic natural membrane environments, providing a tool to investigate interactions with molecules that specifically bind to glycosphingolipids. In addition, biotinylated glycosphingolipids also enable the isolation and purification of specific glycosphingolipid-binding proteins or antibodies using affinity chromatography techniques.
Based on the expertise in the synthesis, purification, and characterization of biotinylated glycosphingolipids, we provide bioconjugation services for biotinylated glycosphingolipids.
We can synthesize biotinylated glycosphingolipids according to specific requirements, including the choice of sphingolipid backbone, carbohydrate residue, and biotin linker. In general, carbohydrate residue is typically activated to facilitate its attachment to the sphingolipid backbone, followed by the biotin molecule combines with to the glycosphingolipid to form biotinylated glycosphingolipids.
Our quality control is performed throughout the project, with product monitoring and analysis at each step of the synthesis. Analytical techniques such as NMR spectroscopy, HPLC-MS can be used to verify the structure and purity of the synthesized biotinylated glycosphingolipids.
Biotinylated glycosphingolipids can be conjugated to proteins or antibodies while retaining their function and specificity for affinity purification, immunoassays, or targeted drug delivery.
Biotinylated glycosphingolipids can be immobilized onto beads, microarrays, or biosensors, for applications like receptor binding studies, cell adhesion assays, or diagnostic tests.
Biotinylated glycosphingolipids can be used to study membrane organization and lipid microdomains, cell adhesion and migration processes, intracellular trafficking, receptor-ligand interactions, affinity purification, diagnostic assays, etc. For example, biotinylated glycosphingolipids are served as a tool for specific detection and quantification of glycosphingolipid-binding molecules. Streptavidin-conjugated enzymes, such as streptavidin-horseradish peroxidase (HRP) or streptavidin-alkaline phosphatase (AP), are linked to the biotinylated glycosphingolipids due to the affinity of streptavidin moiety with biotin, allowing the detection enzyme to be linked to the biotinylated glycosphingolipids. A suitable substrate specific for the conjugated enzyme is added, leading enzyme converts the substrate into a detectable signal, which can be measured spectrophotometrically or fluorometrically. The signal intensity is proportional to the presence or amount of the glycosphingolipid-binding molecules.
