Fluorescence Labeling of Nucleic Acids

Since RNA/DNA does not contain naturally occurring fluorescent groups, and the introduction of fluorescent groups is necessary for fluorescence determination. BOC Sciences has extensive experience in fluorescence chemistry and has developed a wide range of widely used and trusted solutions for fluorescently labeling a wide variety of substances. Fluorescently labeled nucleic acids offer many advantages for gene characterization, quantification, integration and expression studies in molecular biology research, diagnostics and imaging studies.

Fig.1 Fluorescent Labeling of Nucleic Acids. (Rombouts et al., 2016)

What is Fluorescence Labeling of Nucleic Acids?

Fluorescence labeling of nucleic acids is the process of attaching a fluorescent molecule or fluorophore to a nucleic acid molecule to introduce a fluorescent signal. The fluorescent labeling process involves covalent or non-covalent attachment of a fluorescent dye or fluorophore to a nucleic acid. Covalent labeling typically involves attaching a reactive group on the fluorescent molecule to a specific functional group on the nucleic acid, such as an amino or thiol group. Non-covalent labeling methods utilize specific interactions between certain probes and the nucleic acid sequence of interest.

Services for Fluorescently Labeled Nucleic Acids

BOC Sciences offers fluorescent labeling services for nucleic acids, and can perform labeling for both DNA and RNA labeling.

• Fluorescence Labeling of DNA

DNA fluorescent labeling involves binding a fluorescent dye to a DNA molecule in order to track and detect the DNA sequence in an experiment. Our researchers use end-labeling method for labeling, the following are the labeling steps of end-labeling method:

(1) Label the end of the DNA molecule with a fluorescent dye, commonly used markers include fluorescein dyes and so on.

(2) In the 3' end-labeling method, DNA terminal deoxynucleotidyl transferase (TdT) and fluorescent dye-modified reactants (e.g., fluorescein-dUTP) are used for labeling. TdT is able to incorporate fluorescent dye-modified nucleotides on the OH group at the end of the DNA to form fluorescently labeled DNA.

(3) In the 5' end labeling method, several methods can be used, such as the PCR or the Klenow fragmentation reaction. These reactions introduce fluorescent markers at the 5' end of the DNA, such as PCR amplification using fluorescein phosphate and 5' phosphorylated primers.

(4) The labeled DNA can be detected and analyzed by equipment such as gel electrophoresis or fluorescence microscopy.

• Fluorescence Labeling of RNA

BOC Sciences provides reverse transcription labeling, RNA-conjugated dye labeling, fluorescent protein labeling and other methods to combine fluorescent substances with RNA for better application in genetic disease diagnosis, viral infection analysis, prenatal diagnosis, tumor genetics and genomic research.

Fluorescently Labeled Nucleic Acid Detection Technology

Fluorescently labeled nucleic acids can be visualized and detected using fluorescence microscopy, flow cytometry, gel electrophoresis, or other fluorescence-based techniques. The emitted fluorescent signal can provide information about the presence, localization, abundance, or interactions of the labeled nucleic acid, allowing researchers to study various aspects of nucleic acid biology.

Applications of Fluorescent Nucleic Acid Labeling

• Generating information on gene integrity and copy number
• Diagnose specific sequences and chromosomal aberrations
• Simultaneous determination of relative expression of RNAs
• Discover protein-nucleic acid interactions

Our Advantages

• Fluorescence reagent and probe are economical and safe
• Deep knowledge and rich experience in biomaterial modification and conjugation
• The probe is stable and can be used within two years after one labeling
• The experimental period is short, the results can be obtained quickly, the specificity is good, and the location is accurate
• FISH can be located in the DNA sequence of 1kb, and its sensitivity is similar to that of radioactive probe
• Polychromatic FISH can detect multiple sequences at the same time by displaying different colors in the same core
• All samples are carefully monitored for stability and characterized to ensure batch to batch consistency.

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