Fluorescence Labeling of Nucleic Acids

* Please be kindly noted that our services can only be used for research to organizations or companies and not intended for any clinical or individuals.

As a leading service provider in the field of drug discovery and research, BOC Sciences is fully competent and dedicated to serving one-stop molecule labeling services which has be used in various detection and assay system. BOC Sciences provides a variety of solutions for the preparation of fluorescent labeling nucleic acids, including labeling regents, independent dyes and custom labeling services.

Introduction

Fluorescence-based determination is becoming more and more important in the biophysical study of the structure, function and kinetics of nucleic acids. The emergence of single molecule fluorescence spectroscopy provides insight into many of the dynamic and mechanical details previously hidden by integrated techniques. These studies require the stable integration of fluorescent tags into the target system at a specific location. Ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) do not contain natural fluorescent groups. Therefore, in order to carry out fluorescence determination, it is necessary to introduce these groups. Fluorescence labeling of nucleic acids is usually carried out by enzymatic reaction. Organic fluorescent group chemistry was introduced into primers or nucleosides triphosphate, and then amplified by PCR or incorporated into DNA or RNA polymerase or terminal polynucleotides transferase.

Application

Fluorescence labeled nucleotides have many advantages in the study of gene qualitative, quantitative, integration and expression. At present, it has been widely used in many fields, such as genetic disease diagnosis, virus infection analysis, prenatal diagnosis, tumor genetics and genome research. It plays an important role in clinical examination, teaching and research.

Our Advantages

  • Fluorescence reagent and probe are economical and safe
  • Deep knowledge and rich experience in biomaterial modification and conjugation
  • The probe is stable and can be used within two years after one labeling
  • The experimental period is short, the results can be obtained quickly, the specificity is good, and the location is accurate
  • FISH can be located in the DNA sequence of 1kb, and its sensitivity is similar to that of radioactive probe
  • Polychromatic FISH can detect multiple sequences at the same time by displaying different colors in the same core
  • All samples are carefully monitored for stability and characterized to ensure batch to batch consistency.

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