As a professional enzyme labeling service provider, BOC Sciences offers enzyme antibody conjugation services. We offer a wide range of enzymes for antibody labeling, including alkaline phosphatase, horseradish peroxidase, β-galactosidase, etc.
Enzyme conjugated antibodies are made by attaching an enzyme to a specific antibody by appropriate methods. The quality of enzyme-conjugated antibody mainly depends on the enzyme and antibody with good purity, high activity, and high affinity. Once prepared, enzyme-labeled antibodies can be used for a variety of applications including enzyme-linked immunosorbent assay, immunohistochemistry (IHC), protein blotting, and other immunoassays.
Fig.1 Scheme of an antibody modification with enzyme. (Falck et al., 2018)
BOC Sciences can maintain antibody activity by directing the location of the label so that it does not interfere with the antigen binding site. For some conjugates, it may be necessary to modify/purify the antibody or introduce functional groups and linkers to the enzyme or antibody.
For the conjugation of enzymes to antibodies, we typically use heterobifunctional cross-linkers.
The final conjugate first needs to be separated from the excess or unreacted reagent by gel filtration or dialysis. Other purification techniques such as stirred cell filtration, tangential flow filtration (TFF), and gel filtration chromatography can also be used to remove excess reagents or to isolate and characterize the cross-linking product. For reagents that are similar in size to or larger than the antibody, other purification techniques such as affinity chromatography, ion exchange chromatography, and hydrophobic interaction chromatography must be used.
After the purification of the product, many different types of analyses are required, including spectroscopy (MALDI-top, ESI, LC-MS fluorescence), electrophoresis, immunochemical biochemistry, and enzyme analysis. We also independently follow QC (Quality Control) and QA (Quality Assurance) procedures, providing you with the dual assurance that each delivered conjugate is of the highest possible quality.
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