Biotin Labeled Nucleotides
With the comprehensive advanced platform, BOC Sciences provides global clients with biotin labeled nucleotides services to promote your research.
What is Biotin Labeled Nucleotides?
Biotin, also known as vitamin B7, which has a strong affinity for avidin and streptavidin, making it an excellent choice for molecular labeling. Nucleotides are the building blocks of DNA and RNA, consist of a nitrogenous base (adenine, guanine, cytosine, thymine, or uracil), a sugar molecule (ribose or deoxyribose), and a phosphate group. By incorporating biotin in the nucleotide, a label is introduced that can be easily recognized and utilized in molecular biology applications. Biotin labeled nucleotides find extensive applications in areas such as DNA sequencing, gene expression analysis, and nucleic acid detection. Biotin acts as a small, stable and highly specific tag that allows one to easily detect and track the nucleotide sequences it binds. Moreover, the small size of biotin has minimal impact on the structure and function of nucleotides.
How to Synthesize Biotinylated Nucleotides
Biotinylated nucleotides are compounds that are linked to nucleotides through the carboxyl site of biotin. Common strategies for biotinylated nucleotides synthesis include using biotinylating agents that react with functional groups on the nucleotide, such as amino groups or hydroxyl groups. Examples of commonly used biotinylation reagents include NHS-biotin (N-hydroxysuccinimide-biotin) and EDC-biotin (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-biotin). If necessary, protect any sensitive functional groups on the nucleotide that may interfere with the biotinylation reaction.
Applications of Biotin Labeled Nucleotides
DNA Sequencing
Biotin-labeled nucleotides are crucial for DNA sequencing techniques, including Sanger sequencing and next-generation sequencing (NGS). By incorporating biotin-labeled nucleotides into DNA strands, researchers can track DNA synthesis and analyze DNA sequences more efficiently.
In situ Hybridization (ISH)
ISH is a powerful technique used to visualize gene expression patterns and identify specific nucleic acid sequences within cells or tissues. Biotin-labeled nucleotides are employed to label target sequences, which can be detected using streptavidin-conjugated fluorescent probes.
Real-time PCR
Real-time PCR is a widely used method for quantifying gene expression levels. Biotin-labeled nucleotides can be incorporated into PCR amplicons, allowing for subsequent detection using streptavidin-conjugated reporters.
Nucleic Acid Pull-down Assays
Biotin-labeled nucleotides are instrumental in nucleic acid pull-down assays, facilitating the study of protein-DNA interactions. By incorporating biotin-labeled nucleotides into target DNA fragments, researchers can selectively capture interacting proteins for further analysis.
Our Biotin Labeled Nucleotide Services
- Customized Solutions: At BOC Sciences, we offer a comprehensive range of biotin labeled nucleotide services tailored to meet the unique needs of each client.
- High-Quality Products: Our products undergo stringent quality control measures to ensure purity, stability, and compatibility with a variety of biotechnological applications.
- Expert Consultation: With extensive experience in biolabeling techniques, our team of scientists is available to provide expert consultation and guidance at every step of your project.
- Timely Delivery: We understand the importance of meeting project deadlines. BOC Sciences is committed to delivering your biotin labeled nucleotides promptly and efficiently, ensuring that your research progresses smoothly.