Magnetic Beads labeled Antibody
BOC Sciences offers customized magnetic beads labeled antibody services that include the selection of specific antibodies and magnetic beads that are optimized and validated to ensure high specificity and efficiency. BOC Sciences' nanoparticles and beads conjugation services provide a convenient, efficient, and reliable tool for the detection, isolation, and analysis of target molecules, advancing scientific research and drug discovery.
Magnetic Bead Antibody Conjugation
Magnetic beads exhibit superparamagnetic properties in the presence of an external magnetic field, where they can rapidly aggregate and settle. The high binding capacity of magnetic beads facilitates binding to antibodies, and antibody-coated magnetic beads can be used for positive selection or detection of specific antigens. When magnetic bead conjugated antibody bind to a specific antigen, an antigen-antibody-bead immune complex is formed. Antibody coupling to magnetic beads is widely used in immunoassays with the advantages of easy handling, speed and mildness. Under the action of a magnetic field, the complex is separated from the rest of the material to achieve the purpose of separating, purifying and concentrating the specific antigenic material.
Fig.1 Magnetic bead separation of proteins and spermatozoa. (Phiphattanaphiphop et al., 2022)
Antibody Coated Magnetic Beads Services
In order to ensure the success of magnetic bead labeled antibodies, BOC Sciences needs to take into account the planning of the binding protocol, the density of functional groups on the surface of the beads, and the controlled magnetic separation of the beads when designing the assay. Typically, we perform the following labeling steps when labeling antibodies with magnetic beads:
- Initial Cleaning
In this step, the buffer in which the magnetic beads are located is removed as it may contain components that may interfere with binding. The magnetic beads are attracted to the magnetic separator and the initial buffer is removed. When the magnetic bead buffer is removed, the appropriate antibody or antigen binding buffer is added to the magnetic beads.
- Cross Linking Antibody to Magnetic Beads
There are two types of cross-linking: chemical cross-linking and physical adsorption. Chemical cross-linking occurs when a chemical cross-linking agent (Dithiophenol, Glutaraldehyde, etc.) forms a covalent bond with a functional group on the magnetic beads, resulting in a stable antibody-magnetic bead complex. Physical adsorption occurs when the magnetic beads have specific functional groups on the surface, such as amino groups, carboxyl groups, etc., which allow for non-covalent adsorption with the functional groups on the antibody.
The method of cross-linking can be selected according to the properties of the magnetic beads and the antibody itself.
- Blocking and Washing
Blocking causes the free space or free chemical groups on the surface of the magnetic beads to be blocked to prevent non-specific binding. Classical blocking agents are BSA, casein, PEG and glycine, which bind to free radicals on the magnetic beads.
- Final Buffer
The final buffer for magnetic bead binding is added to maintain the stability of the magnetic beads and is also compatible with the immunoassay reaction. It will prevent aggregation of the magnetic beads and help keep the beads stable. To prevent contamination, an antimicrobial agent is added, usually in the range of 0.05 to 0.10%.
The antibody coated magnetic beads are passed through a strong, stable magnetic field in which the cells can be analyzed, and the matrix in the sorting column creates a high gradient magnetic field. Magnetically labeled cells remain in the column while unlabeled cells flow out. When the column is removed from the magnetic field, the magnetically labeled cells that remain in the column can be eluted out, resulting in complete access to both labeled and unlabeled cell fractions.
Applications of Magnetic Beads Labeled Antibody
Magnetic beads antibody conjugates are widely used in biomedical research, biopharmaceuticals, clinical diagnostics and food safety. For example, it can be used to isolate and purify recombinant proteins, antibodies, viruses, cells, organelles and other biomolecules, as well as for the detection and diagnosis of diseases and contaminants in food.
Project Workflow
Reference
- Qiao F Y, et al. Antibody and DNA dual-labeled gold nanoparticles: Stability and reactivity[J]. Applied surface science, 2008, 254(10): 2941-2946.