Biotin Labeled Nucleic Acids

BOC Sciences offers biotin-labeled nucleic acids to clients worldwide with a comprehensive range of state-of-the-art platforms to power your research.

Fig.1 Biotinylation of RNA and DNA. (Lat et al., 2020)

Biotinylated Nucleic Acids

Biotinylation, also known as biotin labeling, is the process of covalently attaching biotin to proteins, nucleic acids, or other molecules. Biotinylation is rapid, specific, and because of the small size of biotin, it is unlikely to interfere with the natural function of the molecule. Biotin-labeled nucleic acids can be used in in situ hybridization and immunohistochemistry to detect and localize the presence and distribution of target nucleic acid sequences in tissues or cells. After hybridization of biotin-labeled nucleic acids to the target nucleic acids, the target nucleic acids can be visualized and detected using streptavidin-enzyme conjugates (e.g., horseradish peroxidase) or fluorescently labeled streptavidin. By biotinylating nucleic acids, molecular specificity, visualization and enrichability of nucleic acids can be conferred, leading to a wide range of applications in biological research, diagnostics and analysis.

Nucleic Acid Biotin Labeling Services

Biotinylated nucleic acids can be customized according to specific customer needs. Customers can choose the appropriate service and work with us to obtain biotin-labeled nucleic acid samples that meet their experimental requirements.

Biotinylated Oligonucleotides

Biotin can be added to either end of the oligonucleotide or internally via modified thymidine residues (biotin-dT). There are several types of biotin modifications, each with its own advantages, all containing the same functional biotin moiety. The size of the DNA or RNA oligonucleotide covalently attached to the biotin appears to limit the ability to bind to immobilized streptavidin, and the ability to capture large DNA fragments with magnetic streptavidin beads is directly correlated with the arm length of the streptavidin. Therefore, we also offer long-chain biotin to enhance binding of large DNA fragments and improve binding kinetics and accessibility of enzymatic events on solid phase surfaces.

Botin Labeled DNA

Biotin-labeled DNA refers to DNA molecules modified with biotin, a small molecule consisting of a vitamin B7 derivative. Addition of biotin to DNA allows for specific binding of streptavidin or affinity proteins that have a high affinity for biotin.

Botin Labeled RNA

Similar to Biotin Labeled DNA, the incorporation of Biotin into RNA allows for specific binding to streptavidin or affinity proteins. Biotin labeling can be achieved by a variety of methods, including enzymatic incorporation, chemical synthesis, or site-specific modification.

Botin Labeled Nucleotides

When a nucleotide is biotinylated (attached with biotin), it gains the ability to bind specifically with molecules that recognize biotin, such as avidin or streptavidin. This binding is highly specific and strong, making biotin-labeled nucleotides useful in various molecular biology applications.

Biotin-labeled dNTPs are standard dNTPs (deoxynucleoside triphosphates) where biotin is conjugated to the nucleoside. The biotin molecule is typically attached to the nucleotide via a linker molecule, ensuring that biotin does not interfere with base pairing or polymerase activity. Biotin-dNTPs are used to incorporate biotin into newly synthesized DNA strands during PCR (polymerase chain reaction) or other DNA synthesis reactions.

Biotin-labeled ddNTPs are modified versions of ddNTPs (dideoxynucleoside triphosphates) with biotin attached. Biotin-ddNTPs are used in sequencing methods (e.g., Sanger sequencing) to terminate DNA synthesis at specific bases, generating a ladder of terminated fragments that can be sequenced.

Analysis of Biotinylated Nucleic Acids

After labeling is complete, the sample undergoes a purification step to remove unreacted reagents and by-products. At any time, we offer services for the analysis and evaluation of labeled nucleic acid samples, including nucleic acid purification, concentration determination, purity assessment and quality control.

Sample Delivery

Upon completion of purification and quality control, samples will be packaged into appropriate forms, such as lyophilized powders or solutions, and ensure proper storage conditions. The samples will then be delivered to the customer via a suitable mode of transportation (e.g. courier).

Application of Nucleic Acid Biotinylation

• Gene Positioning: by in situ hybridization, a specific gene probe labeled with biotin can be used to directly detect tissue sections, specific DNA sequences in individual cells or chromosomes fixed on slides.
• Nucleic acid sequence analysis: using biotin instead of 30P marker for nucleic acid sequence analysis, both chemical degradation and terminal termination have been successfully tried.
• Gene isolation and purification: first, the pure RNA was biotinylated and then hybridized with the total DNA products. Only the DNA molecules that could recognize the RNA could be connected to the Bio RNA. At this time, specific DNA fragments can be enriched by avidin Sepharose affinity adsorption column.
• Identification of plant viruses and viroids: A biotin-labeled nucleic acid probe of a plant virus or virus-like virus was used for dot hybridization with a sample of NCM. Finally, the hybridization results were shown by affinity enzyme-linked assay system.

Our Advantages

• Extensive experience in the fields of enzymology, nucleic acid chemistry and dye chemistry
• Obtaining more stable biotin or streptavidin complex
• Carefully monitored for stability and characterized to ensure batch to batch consistency
• Full spectrum of biotin labeling services

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