Proximity BiotinylationCell Surface LabelingBiotinylation Assay
We specialize in cell biotinylation services that enable researchers to precisely label, track, and analyze proteins on living cells. By combining optimized chemical and enzymatic labeling strategies with strict quality control, we ensure that cell-surface proteins or protein interaction networks are biotin-tagged with high specificity and minimal impact on cell viability. This powerful labeling approach is compatible with downstream workflows such as proteomics, antibody validation, receptor-ligand studies, and drug discovery, giving you accurate and reproducible data to accelerate your research and development.
Cell biotinylation is a powerful tool that addresses critical challenges in modern biology and drug development:
Difficult-to-detect membrane proteins: Enables selective labeling and enrichment of low-abundance surface proteins.
Protein-protein interaction mapping: Facilitates identification of interaction partners through proximity labeling methods (e.g., BioID, APEX).
Target validation in therapeutics: Supports antibody, receptor, and ligand studies by confirming cell-surface expression.
High sensitivity in proteomics workflows: Improves downstream mass spectrometry and affinity-based analyses.
Streamlined cell engineering: Allows precise modification of live cells for imaging, tracking, or therapeutic applications.
By overcoming these barriers, biotinylation helps researchers generate accurate, reproducible data and achieve deeper biological insights.
Cell Surface Biotinylation
Proximity-Dependent Biotinylation (BioID / APEX)
Custom Biotin Conjugation
Sample Preparation & Enrichment
Data Support & Consultation
We start by discussing your research goals, experimental design, and specific labeling requirements to tailor the biotinylation strategy.
Cells or biomolecules are prepared under optimized conditions to ensure viability and compatibility with the chosen biotinylation method.
Using chemical or enzymatic techniques, we perform precise labeling of cell-surface proteins, intracellular interactomes, or custom biomolecules.
Biotinylated targets are isolated using streptavidin-based systems, delivering high-purity material for downstream analysis.
Each project undergoes strict validation steps to confirm labeling efficiency, specificity, and reproducibility.
Final samples and detailed reports are delivered, with optional consultation to integrate results into proteomics, drug discovery, or diagnostic workflows.
Our cell biotinylation service ensures precise labeling of surface proteins or protein interactomes without compromising cell viability. This guarantees reliable results for receptor profiling, antibody validation, and functional studies.
From cell surface labeling to protein interaction mapping, our methods support a wide range of applications including proteomics, biomarker discovery, drug screening, and therapeutic target validation.
We provide custom biotinylation services tailored to your project needs - whether you require chemical conjugation, enzymatic labeling, or proximity-based biotinylation technologies (BioID / APEX).
Our workflows are fully compatible with downstream techniques such as western blotting, ELISA, mass spectrometry, and flow cytometry, enabling smooth integration into your existing research pipeline.
With a team of experienced scientists, we not only execute your project but also guide you through experimental design, optimization, and data interpretation to maximize research impact.
Cell biotinylation is primarily used to label and isolate surface proteins or map protein interactions inside living cells. This makes it highly valuable for proteomics, drug discovery, cell biology, and diagnostics.
We use optimized, non-permeable reagents and protocols that preserve cell viability while ensuring selective labeling. Proximity-labeling approaches are also designed to minimize disruption of cellular functions.
We can work with a variety of sample types, including cultured cell lines, primary cells, recombinant proteins, antibodies, and peptides. Each project is customized to meet your experimental requirements.
After labeling and enrichment, we provide high-purity biotinylated proteins or cell samples, along with a detailed report on labeling efficiency and experimental validation.
Yes. Our service includes optional support for integrating biotinylated samples into workflows such as western blotting, ELISA, flow cytometry, and mass spectrometry.
Unlock the power of cell biotinylation services to advance your proteomics, drug discovery, and diagnostic studies with precision and reliability. Whether you need cell surface labeling, protein interaction mapping, or custom biotin conjugation, our expert team is here to support your project from start to finish. Contact us today for a free consultation or request a customized quote - let's bring your research to the next level.
