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Biotinylated Antigen Services

Biotinylated Antigen Services

Site-Specific BiotinylationCustom Biotinylated Antigen

We specialize in delivering custom biotinylated antigen services tailored to the needs of academic researchers, diagnostic developers, and pharmaceutical companies. By combining state-of-the-art chemical biotinylation techniques with advanced site-specific enzymatic methods such as BirA-mediated Avi-tag labeling, we ensure that each antigen retains its structural integrity and biological activity after conjugation. Our services cover a wide spectrum of biomolecules, including proteins, peptides, and recombinant antigens, providing clients with ready-to-use solutions for immunoassays, biosensor platforms, and vaccine research. With strict quality control, scalable production, and expert consultation, we help you transform your antigen into a powerful tool for sensitive, reliable, and reproducible results.

Problems We Solve

Unreliable antigen labeling

Random or poorly controlled biotinylation can reduce antigen activity and binding specificity. Our optimized workflows maintain native antigen functionality.

Low reproducibility

Many labs struggle with inconsistent labeling efficiency. We guarantee high reproducibility and batch-to-batch consistency.

Limited assay performance

Biotinylated antigens enable strong and specific binding to streptavidin/avidin platforms, boosting assay sensitivity and reducing background noise.

Time & resource constraints

Outsourcing biotinylation saves valuable in-house resources, letting you focus on discovery, validation, and downstream applications.

What We Offer: Protein & Antigen Biotinylation Services

antibodyCustom Biotinylation of Proteins & Peptides
  • Multiple strategies including amine-reactive, thiol-reactive, and enzymatic biotinylation.
  • Flexible labeling densities tailored to your research or diagnostic application.
  • Preservation of antigen structure and biological activity.
  • Customizable solutions for proteins, peptides, and recombinant antigens.
antibodyReady-to-Use Biotinylated Antigens
  • Extensive catalog of biotinylated recombinant proteins across major research areas.
  • Standardized production for consistent batch-to-batch performance.
  • Immediate availability for ELISA, SPR/BLI, and rapid diagnostic testing.
  • Reduced project turnaround time compared to in-house labeling.
antibodyQuality Validation & QC Testing
  • Biotin-to-protein ratio determination for precise characterization.
  • Purity verification via SDS-PAGE, HPLC, or mass spectrometry.
  • Functional validation in binding assays to confirm antigen activity.
  • Comprehensive QC reports included with every order.
antibodyScalable Production
  • Research-scale production starting from milligram quantities.
  • Gram-level manufacturing suitable for diagnostics and preclinical studies.
  • Seamless scale-up from pilot projects to large-scale supply.
  • Consistent quality maintained across different production scales.
antibodyApplication Support
  • Expert consultation on assay design and antigen integration.
  • Technical guidance for ELISA, Western blot, flow cytometry, and IHC.
  • Specialized support for SPR/BLI kinetic binding studies.
  • Troubleshooting assistance to optimize performance and reproducibility.

Workflow for Custom Antigen Biotinylation Services

Expertise
Project Consultation

Our scientific team evaluates your specific needs, antigen type, and intended applications to recommend the most suitable biotinylation strategy.

Customization
Design & Method Selection

Based on your requirements, we choose chemical or enzymatic labeling approaches, ensuring the right balance between efficiency and antigen integrity.

Quality assurance
Biotinylation & Purification

Antigens are conjugated with biotin under optimized conditions, followed by thorough purification to remove excess reagents.

Timely delivery
Quality Control & Validation

Each batch undergoes biotin-to-protein ratio analysis, purity checks, and functional activity assays to confirm reliability.

Collaborative approach
Delivery & Support

Final products are delivered with full QC documentation, and our experts remain available for technical guidance and troubleshooting.

Key Benefits of Biotinylated Antigen Services

Customizable Options
Custom Services

We provide tailored solutions for proteins, peptides, and recombinant antigens, ensuring that each project meets your specific research or diagnostic application.

Customizable Options
Site-Specific Biotinylation

Our enzymatic labeling platform delivers consistent, single-site biotin incorporation, preserving antigen structure and improving assay performance.

Customizable Options
High-Purity

All products undergo stringent purification and validation, guaranteeing high-quality reagents that enhance sensitivity and reproducibility in immunoassays and biosensor studies.

Customizable Options
Scalable Production

From milligram-scale research batches to gram-level manufacturing, we support projects of all sizes with seamless scalability and consistent quality.

Customizable Options
Reliable Antigen Labeling

Comprehensive quality control, including activity testing and detailed QC reports, provides clients with full confidence in the performance of every biotinylated antigen.

Where to Use Biotinylated Antigens?

Immunoassay Development (ELISA, Lateral Flow, Western Blot)
  • Enhanced Sensitivity - Biotinylated antigens improve assay performance through strong streptavidin-biotin binding.
  • High Reproducibility - Consistently labeled antigens deliver reliable results across experiments.
  • Low Background - Optimized labeling reduces non-specific signals for cleaner data.
Biosensor & Kinetic Analysis (SPR, BLI, Biacore, Octet)
  • Precise Immobilization - Site-specific biotinylation ensures accurate antigen orientation on sensor surfaces.
  • Maintained Activity - Advanced labeling preserves antigen structure and binding sites.
  • High-Throughput Screening - Ideal for rapid antibody affinity and kinetics measurements.
Antibody Discovery & Validation
  • Efficient Screening - Biotinylated antigens accelerate monoclonal and polyclonal antibody testing.
  • Reliable Capture - Strong streptavidin-biotin binding enables robust antibody-antigen interaction assays.
  • Epitope Mapping - Supports detailed analysis of antibody specificity and cross-reactivity.
Diagnostic Development & Point-of-Care Testing
  • Validated Reagents - Biotinylated antigens provide ready-to-use components for diagnostic kits.
  • Scalable Production - Consistent quality from research-grade to industrial manufacturing.
  • Faster Time-to-Market - Ready stock and custom services shorten development cycles.
Vaccine Research & Immunology Studies
  • Controlled Antigen Presentation - Biotin-tagged antigens enable precise surface display for immune studies.
  • Enhanced Immunogenicity - Supports testing of vaccine formulations and immune responses.
  • Immune Mechanism Research - Facilitates studies of T-cell, B-cell, and antigen-antibody interactions.

Frequently Asked Questions (FAQs)

What is a biotinylated antigen and why is it useful?

A biotinylated antigen is a protein or peptide chemically or enzymatically labeled with biotin. This allows strong and specific binding to streptavidin or avidin, making it highly valuable in immunoassays, biosensors, and diagnostic platforms.

Do you offer both chemical and site-specific biotinylation?

Yes, we provide flexible options including random chemical labeling (amine- or thiol-reactive) and site-specific enzymatic labeling using Avi-tag with BirA ligase, depending on the project requirements.

Each product undergoes comprehensive QC testing, including biotin-to-protein ratio measurement, purity analysis (SDS-PAGE, HPLC), and activity assays to confirm functionality. Full reports are delivered with every order.

We offer scalable production ranging from milligram quantities for research use to gram-level batches suitable for preclinical and diagnostic development.

Our biotinylated antigens are ideal for ELISA, SPR/BLI kinetic studies, antibody screening, diagnostic kit development, and vaccine research.

Get Started Today

Ready to accelerate your research and product development with custom biotinylated antigen services? Whether you need site-specific Avi-tag biotinylation, high-purity antigens for ELISA or SPR, or scalable protein labeling solutions, our team is here to deliver precision, quality, and speed. Contact us today to request a quote and discover how our expertise in biotinylated antigen production can empower your next breakthrough.

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