Gold Nanoparticles labeled Peptides
Relying on years of experience, BOC Sciences can provide gold nanoparticles in various sizes for labeling peptides. We guarantee that each customized project is strictly monitored according to the quality control standards.
Peptide Gold Nanoparticles
Gold nanoparticle-labeled peptides are produced by combining gold nanoparticles with specific peptide sequences and are used for visualization, detection or localization of cells, tissues or biomolecules. Gold nanoparticle-labeled peptides have unique optical and electronic properties, and their assembly can be triggered by changes in the local environment. An increasing number of studies are applying these properties to biomedical applications, such as the detection of metal ions, enzymes and antibodies.
Fig.1 Gold nanoparticles are stabilized by a peptide amphiphile. (Egorova et al., 2020)
Binding of Gold Nanoparticles with Peptides
- Non-covalent binding: Non-covalent binding utilizes the chemical properties of the surface of gold nanoparticles to have non-covalent interactions with peptides. Such binding modes include electrostatic interactions, hydrogen bonding, hydrophobic interactions, etc.
- Covalent binding: Covalent binding occurs through the introduction of appropriate functional groups on the surface of gold nanoparticles that are covalently bonded to reactive groups in the peptide. Commonly used covalent binding methods include the use of cross-linking agents to react with sulfhydryl groups on the surface of gold nanoparticles to form reversible or irreversible covalent bonds.
- Affinity binding: It utilizes the affinity of biomolecules, such as the extraordinarily high affinity of biotin-streptavidin, to bind gold nanoparticles to peptides.
Gold Nanoparticle Labeled Peptide Services
BOC Sciences offers customized services for labeling peptides with gold nanoparticles, including ultra-small gold particles, and our researchers will select the optimal labeling strategy
| Labeling Services | Specification |
| Gold Nanoparticle Labeled Signal Peptides | Signal peptides affect cellular function and behavior by binding to specific receptors on the cell surface and triggering a series of signal transduction pathways. BOC Sciences offers solutions for visualizing and detecting signal peptides by combining gold nanoparticles with signal peptides. |
| Gold Nanoparticle Labeled Neuropeptides | Neuropeptides are short peptides that play a role in regulating and transmitting information in the nervous system. Neuropeptides bind to the modified gold nanoparticles through covalent or non-covalent bonds, and are stably attached to the surface of gold nanoparticles. |
| Gold Nanoparticle Labeled Antimicrobial Peptide | BOC Sciences designs gold nanoparticle labeling experiments based on the antimicrobial peptides supplied by our clients. Our technicians will then use advanced technology to combine the gold nanoparticles with the antimicrobial peptide, and measure the performance of the product after completion to ensure that it is as effective as the customer requires. |
| Gold Nanoparticle Labeled Cell-penetrating Peptide | Cell-penetrating peptides (CPPs) are a class of short peptides that are able to cross cell membranes or tissue barriers. depending on the needs of the customer, BOC Sciences can modify gold nanoparticles so that they can bind better to cell-penetrating peptides. Subsequently, we provide cell penetration assays to evaluate the localization and effect of the gold nanoparticle-labeled cell-penetrating peptide within the cell. |
Characterization of Gold Nanoparticle Labeled Peptides
Characterization of gold nanoparticle-labeled peptides can be performed using ultraviolet-visible (UV-Vis) absorption spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and electrochemical measurements.
Reference
- Egorova E A, et al. One peptide for them all: gold nanoparticles of different sizes are stabilized by a common peptide amphiphile[J]. ACS nano, 2020, 14(5): 5874-5886.